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Literature summary for 6.3.1.2 extracted from

  • Antonyuk, L.P.
    Glutamine synthetase of the rhizobacterium Azospirillum brasilense: Specific features of catalysis and regulation (2007), Appl. Biochem. Microbiol., 43, 244-249.
No PubMed abstract available
Search for more literature for 6.3.1.2

Activating Compound

Activating Compound Comment Organism Structure
glutamate in the presence of Mg2+ the activity increases with the concentration of the substrate Azospirillum brasilense
NH4+ in the presence of Mg2+ the activity increases with the concentration of the substrate Azospirillum brasilense

Inhibitors

Inhibitors Comment Organism Structure
L-alanine decreases the enzyme activity considerably Azospirillum brasilense
L-arginine inhibitory at high concentrations Azospirillum brasilense
L-glutamate in the presence of Mn2+ the activity decreases when exceeding a concentration of 10 mM glutamate Azospirillum brasilense
L-glutamine inhibitory at high concentrations Azospirillum brasilense
L-Glycine decreases the enzyme activity considerably Azospirillum brasilense
L-serine decreases the enzyme activity considerably Azospirillum brasilense
NH4+ in the presence of Mn2+ the activity decreases when exceeding a concentration of 20 mM NH4+ Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 L-Glu in the presence of Mg2+ Azospirillum brasilense
0.042
-
 NH4+ in the presence of Mg2+ Azospirillum brasilense
0.2
-
 ATP in the presence of Mg2+ Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ supports the enzymatic activity Azospirillum brasilense
Mg2+ supports the enzymatic activity Azospirillum brasilense
Mn2+ supports the enzymatic activity Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Azospirillum brasilense ADP + phosphate + L-Gln
-
 ?

Subunits

Subunits Comment Organism
dodecamer type alpha12 dodecamer with oligomer and monomer having molecular weights of 630000 and 52000 Da, respectively Azospirillum brasilense

Synonyms

Synonyms Comment Organism
GSI
-
 Azospirillum brasilense

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.25
-
 in the presence of Mn2+, the enzyme is active within a relatively narrow range of weakly acidic pH (no higher than 6.25) and inactive at alkaline pH Azospirillum brasilense
6.5 9.75 with Mg2+ GS is active within a broad range of physiological pH values (6.5-9.75) Azospirillum brasilense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.25
-
 in the presence of Mn2+, at optimum pH values, moderately adenylated GS is twice more active in the presence of Mn2+ (at pH 6.25) than Mg2+ (at pH 8.0), whereas, at pH 7.1, which corresponds to the intracellular level of pH for the enzyme is almost equiactive with either cation Azospirillum brasilense
8
-
 in the presence of Mg2+, at optimum pH values, moderately adenylated GS is twice more active in the presence of Mn2+ (at pH 6.25) than Mg2+ (at pH 8.0), whereas, at pH 7.1, which corresponds to the intracellular level of pH for the enzyme is almost equiactive with either cation Azospirillum brasilense