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Literature summary for 6.3.1.2 extracted from
- Purification and properties of glutamine synthetase from the non-N2-fixing cyanobacterium Phormidium laminosum (1989), J. Bacteriol., 171, 1158-1165.
General Stability
| General Stability | Organism |
|---|---|
| the presence of Mn2+ or Mg2+ drastically affects the thermostability of transferase activity and biosynthetic activity | Phormidium laminosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | biosynthetic activity | Phormidium laminosum |  |
| Ala | - |
Phormidium laminosum | |
| AMP | biosynthetic activity | Phormidium laminosum | |
| Asp | - |
Phormidium laminosum | |
| Gly | - |
Phormidium laminosum | |
| L-Arg | biosynthetic activity | Phormidium laminosum | |
| L-Glu | inhibition of transferase activity, no inhibition of biosynthetic activity | Phormidium laminosum | |
| L-Lys | - |
Phormidium laminosum | |
| L-Met | - |
Phormidium laminosum | |
| L-methionine sulfoximine | - |
Phormidium laminosum | |
| L-Tyr | inhibits transferase activity, no inhibition of biosynthetic activity | Phormidium laminosum | |
| p-hydroxymercuribenzoate | biosynthetic activity | Phormidium laminosum | |
| Ser | - |
Phormidium laminosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | partially effective as activator of biosynthetic activity and transferase activity, half-maximal activation of biosynthetic reaction at 6.3 mM | Phormidium laminosum | |
| Cu2+ | partially effective as activator of transferase activity | Phormidium laminosum | |
| Mg2+ | essential activator for biosynthetic activity, half-maximal activation at 18 mM. Partially effective as activator of transferase activity | Phormidium laminosum | |
| Mn2+ | required for transferase activity | Phormidium laminosum | |
| Zn2+ | partially effective as activator of biosynthetic activity, half-maximal activation at 6.3 mM | Phormidium laminosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 602000 | - |
gel filtration | Phormidium laminosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phormidium laminosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phormidium laminosum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine | ordered three-reactant system with the binding order: NH4+, L-Glu and ATP | Phormidium laminosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Phormidium laminosum |
| 4.4 | - |
biosynthetic assay | Phormidium laminosum |
| 152 | - |
gamma-glutamylhydroxamate assay | Phormidium laminosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Phormidium laminosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-Glu + NH4+ | - |
Phormidium laminosum | ADP + phosphate + L-Gln | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | SDS-PAGE of 2-mercaptoethanol-treated enzyme | Phormidium laminosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
transferase activity | Phormidium laminosum |
| 55 | - |
biosynthetic activity | Phormidium laminosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Phormidium laminosum |
| 7.5 | - |
transferase activity | Phormidium laminosum |
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