General Stability | Organism |
---|---|
ATP decreases stability if present with lower concentrations of Mg2+ or Mn2+. When Mg2+ or Mn2+ is present in 4 mM excess over ATP, ATP slightly increases stability | Pisum sativum |
partially stabilized by divalent cation, Mg2+ or Mn2+, and by 0.5-1.5 M sucrose, 1-2.5 M fructose or 20-30% ethyleneglycol | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
shoot | of light-grown seedlings | Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.9 | - |
gamma-glutamylhydroxamate assay | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Pisum sativum | ADP + phosphate + L-Gln | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the pH-optimum varies from pH 7.3-7.4 to pH 8.2-8.6 depending on the Mg2+ concentration | Pisum sativum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.2 | optimal stability varies widely, pH 5.0-8.2, depending on the type and concentration of divalent cation, Mn2+, Co2+, Mg2+ and the ATP concentration | Pisum sativum |