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Literature summary for 6.3.1.2 extracted from

  • O¦Neal, D.; Joy, K.W.
    Glutamine synthetase of pea leaves. I. Purification, stabilization, and pH optima (1973), Arch. Biochem. Biophys., 159, 113-122.
    View publication on PubMed
Search for more literature for 6.3.1.2

General Stability

General Stability Organism
ATP decreases stability if present with lower concentrations of Mg2+ or Mn2+. When Mg2+ or Mn2+ is present in 4 mM excess over ATP, ATP slightly increases stability Pisum sativum
partially stabilized by divalent cation, Mg2+ or Mn2+, and by 0.5-1.5 M sucrose, 1-2.5 M fructose or 20-30% ethyleneglycol Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pisum sativum
-
shoot of light-grown seedlings Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.9
-
 gamma-glutamylhydroxamate assay Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Pisum sativum ADP + phosphate + L-Gln
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the pH-optimum varies from pH 7.3-7.4 to pH 8.2-8.6 depending on the Mg2+ concentration Pisum sativum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.2 optimal stability varies widely, pH 5.0-8.2, depending on the type and concentration of divalent cation, Mn2+, Co2+, Mg2+ and the ATP concentration Pisum sativum