Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala | - |
Methylococcus capsulatus | |
AMP | - |
Methylococcus capsulatus | |
Gly | - |
Methylococcus capsulatus | |
His | weak | Methylococcus capsulatus | |
L-Gln | - |
Methylococcus capsulatus | |
Mg2+ | addition of Mg2+ to the Mn2+-dependent transferase activity | Methylococcus capsulatus | |
Ser | - |
Methylococcus capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
ADP | - |
Methylococcus capsulatus | |
0.6 | - |
ATP | - |
Methylococcus capsulatus | |
0.72 | - |
NH4+ | - |
Methylococcus capsulatus | |
2.25 | - |
Glu | - |
Methylococcus capsulatus | |
14 | - |
Glu | hydroxylamine, glutamyl transferase assay | Methylococcus capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation required, specificity for Mg2+ in the biosynthetic assay | Methylococcus capsulatus | |
Mn2+ | supports activity of adenylylated and deadenylylated enzyme, no other divalent cation can support gamma-glutamyl transferase activity, optimal concentration is 0.3 mM | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
10 * 60000, SDS-PAGE | Methylococcus capsulatus |
617000 | - |
gel filtration | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
306 | - |
- |
Methylococcus capsulatus |
Storage Stability | Organism |
---|---|
25 mM imidazole/HCl buffer, pH 7.15, 5 mM MgCl, 10 mM sodium glutamate, 5 mM 2-mercaptoethanol and 1 mM EDTA, at -80°C completely stable for several months, at 4°C stable for several hours | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Methylococcus capsulatus | ADP + phosphate + L-Gln | - |
? | |
Gln + hydroxylamine + ADP | - |
Methylococcus capsulatus | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
GTP + L-Glu + NH4+ | 59% of the activity relative to ATP | Methylococcus capsulatus | GDP + phosphate + L-Gln | - |
? | |
ITP + L-Glu + NH4+ | 32% of the activity relative to ATP | Methylococcus capsulatus | IDP + phosphate + L-Gln | - |
? | |
TTP + L-Glu + NH4+ | 10% of the activity relative to ATP | Methylococcus capsulatus | TDP + phosphate + L-Gln | - |
? | |
UTP + L-Glu + NH4+ | 10% of the activity relative to ATP | Methylococcus capsulatus | UDP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 60000, SDS-PAGE | Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
gamma-glutamyl transferase assay | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Methylococcus capsulatus |