Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.1.2 extracted from

  • Murrell, J.C.; Dalton, H.
    Purification and properties of glutamine synthetase from Methylococcus capsulatus (1983), J. Gen. Microbiol., 129, 1187-1196.
No PubMed abstract available
Search for more literature for 6.3.1.2

Inhibitors

Inhibitors Comment Organism Structure
Ala
-
 Methylococcus capsulatus
AMP
-
 Methylococcus capsulatus
Gly
-
 Methylococcus capsulatus
His weak Methylococcus capsulatus
L-Gln
-
 Methylococcus capsulatus
Mg2+ addition of Mg2+ to the Mn2+-dependent transferase activity Methylococcus capsulatus
Ser
-
 Methylococcus capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 ADP
-
 Methylococcus capsulatus
0.6
-
 ATP
-
 Methylococcus capsulatus
0.72
-
 NH4+
-
 Methylococcus capsulatus
2.25
-
 Glu
-
 Methylococcus capsulatus
14
-
 Glu hydroxylamine, glutamyl transferase assay Methylococcus capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, specificity for Mg2+ in the biosynthetic assay Methylococcus capsulatus
Mn2+ supports activity of adenylylated and deadenylylated enzyme, no other divalent cation can support gamma-glutamyl transferase activity, optimal concentration is 0.3 mM Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 10 * 60000, SDS-PAGE Methylococcus capsulatus
617000
-
 gel filtration Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
306
-
-
 Methylococcus capsulatus

Storage Stability

Storage Stability Organism
25 mM imidazole/HCl buffer, pH 7.15, 5 mM MgCl, 10 mM sodium glutamate, 5 mM 2-mercaptoethanol and 1 mM EDTA, at -80°C completely stable for several months, at 4°C stable for several hours Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Methylococcus capsulatus ADP + phosphate + L-Gln
-
 ?
Gln + hydroxylamine + ADP
-
 Methylococcus capsulatus gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
GTP + L-Glu + NH4+ 59% of the activity relative to ATP Methylococcus capsulatus GDP + phosphate + L-Gln
-
 ?
ITP + L-Glu + NH4+ 32% of the activity relative to ATP Methylococcus capsulatus IDP + phosphate + L-Gln
-
 ?
TTP + L-Glu + NH4+ 10% of the activity relative to ATP Methylococcus capsulatus TDP + phosphate + L-Gln
-
 ?
UTP + L-Glu + NH4+ 10% of the activity relative to ATP Methylococcus capsulatus UDP + phosphate + L-Gln
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 60000, SDS-PAGE Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 gamma-glutamyl transferase assay Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Methylococcus capsulatus