Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | Mycobacterium tuberculosis | key enzyme in the Pup tagging (i.e. pupylation) system. Protein pupylation can be regulated at the enzyme level via an allosteric mechanism | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? | |
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | Mycobacterium tuberculosis ATCC 25618 | key enzyme in the Pup tagging (i.e. pupylation) system. Protein pupylation can be regulated at the enzyme level via an allosteric mechanism | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNU7 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WNU7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein PanB]-L-lysine | a pupylation product with a molecular mass approximately 7 kDa heavier than PanB suggests that each PanB monomer is singly pupylated by PafA. PanB is pupylated primarily on either one of two lysines, namely Lys34 or Lys36. Pupylation on Lys137 is also detected, although less frequently | Mycobacterium tuberculosis | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein PanB]-L-lysine | - |
? | |
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein PanB]-L-lysine | a pupylation product with a molecular mass approximately 7 kDa heavier than PanB suggests that each PanB monomer is singly pupylated by PafA. PanB is pupylated primarily on either one of two lysines, namely Lys34 or Lys36. Pupylation on Lys137 is also detected, although less frequently | Mycobacterium tuberculosis ATCC 25618 | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein PanB]-L-lysine | - |
? | |
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | key enzyme in the Pup tagging (i.e. pupylation) system. Protein pupylation can be regulated at the enzyme level via an allosteric mechanism | Mycobacterium tuberculosis | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? | |
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | PafA is an allosteric enzyme that binds its target substrates cooperatively. In contrast, binding of deamidated Pup (Pup-L-glutamate) by PafA follows simple kinetics and is, therefore, not involved in the allosteric transitions of PafA. Pup and target substrate binding by PafA are independent events | Mycobacterium tuberculosis | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? | |
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | key enzyme in the Pup tagging (i.e. pupylation) system. Protein pupylation can be regulated at the enzyme level via an allosteric mechanism | Mycobacterium tuberculosis ATCC 25618 | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? | |
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | PafA is an allosteric enzyme that binds its target substrates cooperatively. In contrast, binding of deamidated Pup (Pup-L-glutamate) by PafA follows simple kinetics and is, therefore, not involved in the allosteric transitions of PafA. Pup and target substrate binding by PafA are independent events | Mycobacterium tuberculosis ATCC 25618 | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PafA | ambiguous | Mycobacterium tuberculosis |
Pup ligase | - |
Mycobacterium tuberculosis |
ubiquitin-like protein ligase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
[prokaryotic ubiquitin-like protein]-L-glutamate | pH 8.0, 30°C | Mycobacterium tuberculosis | |
0.023 | - |
[protein PanB]-L-lysine | pH 8,0, 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | in Mycobacterium tuberculosis Pup tagging is important for virulence | Mycobacterium tuberculosis |