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Literature summary for 6.3.1.14 extracted from
- Decoding the biosynthesis and function of diphthamide, an enigmatic modification of translation elongation factor 2 (EF2) (2014), Microb. Cell, 1, 203-205 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DPH6, the gene is identified through mining the DPH1-DPH5 interaction network | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + diphthine-[translation elongation factor 2] + NH3 | Saccharomyces cerevisiae | - |
AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
| ATP + diphthine-[translation elongation factor 2] + NH3 | Saccharomyces cerevisiae ATCC 204508 / S288c | - |
AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | Q12429 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 / S288c | Q12429 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + diphthine-[translation elongation factor 2] + NH3 | - |
Saccharomyces cerevisiae | AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
| ATP + diphthine-[translation elongation factor 2] + NH3 | - |
Saccharomyces cerevisiae ATCC 204508 / S288c | AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diphthamide synthetase | - |
Saccharomyces cerevisiae |
| DPH6 | - |
Saccharomyces cerevisiae |
| YLR143w | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a dph6 deletion strain specifically accumulates the diphthine-modified form of EF2, and shows a failure to complete diphthine amidation resulting in loss of ADP ribosylation acceptor activity of EF2 in the presence of diphtheria toxin in vitro. The amidation defect partially protects against DT in vivo and correlates with resistance to EF2 inactivation and growth inhibition by sordarin, collectively traits typical of bona fide diphthamide synthesis mutants | Saccharomyces cerevisiae |
| metabolism | the amidation step of diphthamide biosynthesis in yeast requires DPH6, diphthamide synthesis pathway of budding yeast, overview | Saccharomyces cerevisiae |
| physiological function | Dph6 is an ATP-dependent diphthamide synthetase that catalyses the reaction using ammonium as a cofactor. Diphthamide is a highly conserved modification of archaeal and eukaryal translation elongation factor 2. Dph6 is the catalytically relevant amidase in the amidation step of diphthamide synthesis | Saccharomyces cerevisiae |
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