Application | Comment | Organism |
---|---|---|
drug development | MshC may represent a novel target for new classes of antituberculars | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
gene mshC, construction of three N-terminal-MshC fusion proteins where N-terminal tags include the B1 domain of 1. streptococcal protein G to give GB1-MshC, 2. glutathione-S-transferase to give GST-MshC, and 3. maltose binding protein to give MBP-MshC, for expression in M. smegmatis, expression in enzyme-deficient mutant strain mc2155, i.e. I64 L205P, optimization of recombinant enzyme expression, overview | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis | key enzyme in the biosynthesis of mycothiol, pathway overview | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged MshC and recombinant maltose binding protein-MshC by affinity chromatography on glutathione and amylose resins, respectively | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mycolicibacterium smegmatis |
0.009 | - |
purified recombinant GST-tagged MshC | Mycolicibacterium smegmatis |
0.025 | - |
purified recombinant maltose binding protein-MshC | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | key enzyme in the biosynthesis of mycothiol, pathway overview | Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MshC | - |
Mycolicibacterium smegmatis |
mycothiol ligase | - |
Mycolicibacterium smegmatis |