Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential target for drugs directed against tuberculosis | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene mshC, expression in the mshC-deficient mutant strain I64 of Mycobacterium smegmatis, expression as insoluble protein in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | screening for inhibitor molecules | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycobacterium tuberculosis | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycobacterium tuberculosis Rv2130c | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene mshC | - |
Mycobacterium tuberculosis Rv2130c | - |
gene mshC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from mshC-deficient mutant strain I64 of Mycobacterium smegmatis by ammonium sulfate fractionation, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
spectrophotometric assay method development with coupling of diphosphatase to convert diphosphate to phosphate and spectrophotometric detection of the latter via the phosphomolybdate complex with malachite green, overview | Mycobacterium tuberculosis |
0.15 | - |
purified recombinant enzyme | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycobacterium tuberculosis Rv2130c | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | the ATP-dependent ligation of Cys to GlcNIns is a key step in mycothiol biosynthesis, catalyzed by MshC, which is essential for growth of Mycobacterium tuberculosis | Mycobacterium tuberculosis Rv2130c | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-cysteine:1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase | - |
Mycobacterium tuberculosis |
MshC | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |