Application | Comment | Organism |
---|---|---|
pharmacology | enzyme is a traget for drug development against actinomycetes | Mycolicibacterium smegmatis |
pharmacology | enzyme is a traget for drug development against actinomycetes | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme | Mycobacterium tuberculosis |
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as N-terminally His-tagged enzyme | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-cysteine | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
0.072 | - |
1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside | pH 7.5, 37°C | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycobacterium tuberculosis | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis mc(2)155 | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene mshC, formerly gene cys2 | - |
Mycolicibacterium smegmatis | - |
gene mshC, formerly gene cys2, 2 different splicing variants due to a second start codon | - |
Mycolicibacterium smegmatis mc(2)155 | - |
gene mshC, formerly gene cys2, 2 different splicing variants due to a second start codon | - |
Purification (Comment) | Organism |
---|---|
2400fold, 2 peaks | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.054 | - |
purified enzyme | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | Mycobacterium tuberculosis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis mc(2)155 | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | enzyme is important in the biosynthesis of mycothiol, which plays a role in protecting bacteria against oxidative stress | Mycolicibacterium smegmatis mc(2)155 | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cys:GlcN-Ins ligase | - |
Mycolicibacterium smegmatis |
Cys:GlcN-Ins ligase | - |
Mycobacterium tuberculosis |
More | enzyme is related to class I cysteinyl-tRNA synthetase | Mycolicibacterium smegmatis |
More | enzyme is related to class I cysteinyl-tRNA synthetase | Mycobacterium tuberculosis |
MshC ligase | - |
Mycolicibacterium smegmatis |
MshC ligase | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Mycolicibacterium smegmatis | |
ATP | dependent on | Mycobacterium tuberculosis |