Application | Comment | Organism |
---|---|---|
medicine | the enzyme appears as an attractive target for the development of narrow spectrum antibiotics active against multiresistant Enterococcus faecium | Enterococcus faecium |
Cloned (Comment) | Organism |
---|---|
- |
Enterococcus faecium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Enterococcus faecium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-aspartate + [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n | Enterococcus faecium | the enzyme is responsible for the addition of D-aspartic acid onto the peptidoglycan precursor. specifically ligates the beta-carboxylate of D-Asp to the epsilon-amino group of L-Lys in the nucleotide precursor UDP-N-acetylmuramyl-pentapeptide | [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-aspartate + [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n | the enzyme is responsible for the addition of D-aspartic acid onto the peptidoglycan precursor. specifically ligates the beta-carboxylate of D-Asp to the epsilon-amino group of L-Lys in the nucleotide precursor UDP-N-acetylmuramyl-pentapeptide | Enterococcus faecium | [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate | - |
? | |
ATP + D-aspartate + [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n | the enzyme is highly specific for D-aspartate, as L-aspartate, D-glutamate, D-alanine, D-iso-asparagine and D-malic acid are not substrates | Enterococcus faecium | [beta-GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate | - |
? |