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Literature summary for 6.2.2.3 extracted from

  • McIntosh, J.A.; Schmidt, E.W.
    Marine molecular machines heterocyclization in cyanobactin biosynthesis (2010), ChemBioChem, 11, 1413-1421 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.2.3

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol presence is absolutely required uncultured Prochloron sp. 06037A

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli uncultured Prochloron sp. 06037A

Inhibitors

Inhibitors Comment Organism Structure
AEBSF complete inhibition at 10 mM uncultured Prochloron sp. 06037A
PMSF 1 mM, strong inhibition uncultured Prochloron sp. 06037A

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 [TruE2]-L-amino acyl-L-cysteine pH 8.0, 34°C uncultured Prochloron sp. 06037A

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ minimum Mg2+ concentration that supports catalysis is 1 mM uncultured Prochloron sp. 06037A
Zn2+ binding stoichiometry of the enzyme-metal complex is about 1 mol of Zn per mol of enzyme uncultured Prochloron sp. 06037A

Organism

Organism UniProt Comment Textmining
uncultured Prochloron sp. 06037A B2KYG8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [TruE2]-L-amino acyl-L-cysteine
-
 uncultured Prochloron sp. 06037A ADP + phosphate + [TruE2]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline
-
 ?
ATP + [TruE4]-L-amino acyl-L-cysteine
-
 uncultured Prochloron sp. 06037A ADP + phosphate + [TruE4]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline
-
 ?
additional information presence of ATP is absolutely required. During the course of the reaction, ATP is hydrolyzed to ADP uncultured Prochloron sp. 06037A ?
-
-

Synonyms

Synonyms Comment Organism
TruD
-
 uncultured Prochloron sp. 06037A

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information a TruE2 turnover number of about 0.8 per enzyme and min, or a heterocycle turnover number of about 1.6 per enzyme and min is calculated, pH 8.0, 34°C uncultured Prochloron sp. 06037A

General Information

General Information Comment Organism
physiological function during biosynthesis, the leader sequence, which PatA cleaves, is required in cis for modification by PatD uncultured Prochloron sp. 06037A