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Literature summary for 6.2.2.2 extracted from

  • McIntosh, J.A.; Schmidt, E.W.
    Marine molecular machines heterocyclization in cyanobactin biosynthesis (2010), ChemBioChem, 11, 1413-1421 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.2.2

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol presence is absolutely required Prochloron didemni
dithiothreitol required for heterocyclization reaction uncultured Prochloron sp. 06037A
dithiothreitol required for heterocyclization reaction Prochloron didemni
Mg2+ required for heterocyclization reaction, minimum Mg2+ concentration that supports catalysis is 1 mM uncultured Prochloron sp. 06037A
Mg2+ required for heterocyclization reaction, minimum Mg2+ concentration that supports catalysis is 1 mM Prochloron didemni
Zn2+ protein is strongly associated with Zn even after extensive dialysis, with roughly 1 mol of Zn per mol of enzyme uncultured Prochloron sp. 06037A
Zn2+ protein is strongly associated with Zn even after extensive dialysis, with roughly 1 mol of Zn per mol of enzyme Prochloron didemni

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Prochloron didemni

Inhibitors

Inhibitors Comment Organism Structure
AEBSF 10 min, complete inhibition; complete inhibition at 10 mM Prochloron didemni
AEBSF 10 min, complete inhibition uncultured Prochloron sp. 06037A
PMSF 1 mM, strong inhibition Prochloron didemni
PMSF 1 mM, strong inhibition uncultured Prochloron sp. 06037A

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 [TruE2]-(L-amino acyl-L-cysteine) 34°C, pH 8.0 uncultured Prochloron sp. 06037A
0.3
-
 ATP 34°C, pH 8.0 uncultured Prochloron sp. 06037A

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ minimum Mg2+ concentration that supports catalysis is 1 mM Prochloron didemni
Zn2+ binding stoichiometry of the enzyme-metal complex is about 1 mol of Zn per mol of enzyme Prochloron didemni

Organism

Organism UniProt Comment Textmining
Prochloron didemni Q52QI6
-
-
uncultured Prochloron sp. 06037A B2KYG8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [TruE2]-(L-amino acyl-L-cysteine)
-
 Prochloron didemni ADP + phosphate + [TruE2]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline
-
 ?
ATP + [TruE2]-(L-amino acyl-L-cysteine)
-
 uncultured Prochloron sp. 06037A ADP + phosphate + [TruE2]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline
-
 ?
ATP + [TruE2]-(L-amino acyl-L-threonine)
-
 Prochloron didemni ADP + phosphate + [TruE2]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-5-methyl-2-oxazoline
-
 ?
ATP + [TruE4]-(L-amino acyl-L-cysteine)
-
 Prochloron didemni ADP + phosphate + [TruE4]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline
-
 ?
additional information presence of ATP is absolutely required. During the course of the reaction, ATP is hydrolyzed to ADP Prochloron didemni ?
-
 ?
additional information the precursor peptide leaves the enzyme after the second heterocyclization and before the third heterocyclization Prochloron didemni ?
-
 ?

Synonyms

Synonyms Comment Organism
PatD
-
 Prochloron didemni
TruD
-
 uncultured Prochloron sp. 06037A

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.043
-
 ATP 34°C, pH 8.0 uncultured Prochloron sp. 06037A

General Information

General Information Comment Organism
physiological function during biosynthesis, the leader sequence, which PatA cleaves, is required in cis for modification by PatD Prochloron didemni