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Literature summary for 6.2.1.B11 extracted from

  • Awano, T.; Wilming, A.; Tomita, H.; Yokooji, Y.; Fukui, T.; Imanaka, T.; Atomi, H.
    Characterization of two members among the five ADP-forming acyl coenzyme A (acyl-CoA) synthetases reveals the presence of a 2-(imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis (2014), J. Bacteriol., 196, 140-147.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of TK0944 and TK0943 in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
Isobutyrate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.132 mMs Thermococcus kodakarensis
additional information
-
thioglycolate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.322 mM Thermococcus kodakarensis
additional information
-
phenylacetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.329 mM Thermococcus kodakarensis
additional information
-
acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 1.5 mM Thermococcus kodakarensis
0.0056
-
CoA pH 6.5, 55°C Thermococcus kodakarensis
0.0556
-
ATP pH 6.5, 55°C Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27148
-
2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence Thermococcus kodakarensis
51861
-
2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JIA8 and Q5JIA9 Q5JIA8: alpha-subunit, Q5JIA9: beta-subunit
-

Purification (Commentary)

Purification (Comment) Organism
recombinant TK0944/TK0943 protein Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-(4-hydroxyphenyl)acetate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 2-(4-hydroxyphenyl)acetyl-CoA
-
?
ATP + 2-(indol-3-yl)acetate + CoA low activity. Low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 2-(indol-3-yl)acetyl-CoA
-
?
ATP + 2-methylbutyrate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 2-methylbutyryl-CoA
-
?
ATP + 3-methylthiopropionate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 3-methylthiopropionyl-CoA
-
?
ATP + 4-aminobutyrate + CoA low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 4-aminobutyryl-CoA
-
?
ATP + acetate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + acetyl-CoA
-
?
ATP + glycolate + CoA low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + glycolyl-CoA
-
?
ATP + isobutyrate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + isobutyryl-CoA
-
?
ATP + isovalerate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + isovaleryl-CoA
-
?
ATP + lactate + CoA low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + lactoyl-CoA
-
?
ATP + phenylacetate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + phenylacetyl-CoA
-
?
ATP + thioglycolate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + thioglycolyl-CoA
-
?
additional information activity with 2-(imidazol-4-yl)acetate is less than 5% of the activity with acetate Thermococcus kodakarensis ?
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
ACSIIITk
-
Thermococcus kodakarensis
TK0944/TK0943 protein
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
assay at Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
half-life of the TK2127/TK0943 protein: 380 min Thermococcus kodakarensis
90
-
half-life of the TK2127/TK0943 protein: 170 min Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
thioglycolate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 26.1/s Thermococcus kodakarensis
additional information
-
phenylacetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 40.6/s Thermococcus kodakarensis
additional information
-
acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 49.3/s Thermococcus kodakarensis
additional information
-
Isobutyrate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 50.9/s Thermococcus kodakarensis
53.1
-
CoA pH 6.5, 55°C Thermococcus kodakarensis
53.7
-
ATP pH 6.5, 55°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
phenylacetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 123/s*mM Thermococcus kodakarensis
additional information
-
acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 32.8/s*mM Thermococcus kodakarensis
additional information
-
Isobutyrate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 385/s*mM Thermococcus kodakarensis
additional information
-
thioglycolate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 81.0/s*mM Thermococcus kodakarensis
965
-
ATP pH 6.5, 55°C Thermococcus kodakarensis
9490
-
CoA pH 6.5, 55°C Thermococcus kodakarensis