Cloned (Comment) | Organism |
---|---|
coexpression of TK0944 and TK0943 in Escherichia coli | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Isobutyrate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.132 mMs | Thermococcus kodakarensis | |
additional information | - |
thioglycolate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.322 mM | Thermococcus kodakarensis | |
additional information | - |
phenylacetate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.329 mM | Thermococcus kodakarensis | |
additional information | - |
acetate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 1.5 mM | Thermococcus kodakarensis | |
0.0056 | - |
CoA | pH 6.5, 55°C | Thermococcus kodakarensis | |
0.0556 | - |
ATP | pH 6.5, 55°C | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27148 | - |
2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence | Thermococcus kodakarensis |
51861 | - |
2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JIA8 and Q5JIA9 | Q5JIA8: alpha-subunit, Q5JIA9: beta-subunit | - |
Purification (Comment) | Organism |
---|---|
recombinant TK0944/TK0943 protein | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-(4-hydroxyphenyl)acetate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + 2-(4-hydroxyphenyl)acetyl-CoA | - |
? | |
ATP + 2-(indol-3-yl)acetate + CoA | low activity. Low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + 2-(indol-3-yl)acetyl-CoA | - |
? | |
ATP + 2-methylbutyrate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + 2-methylbutyryl-CoA | - |
? | |
ATP + 3-methylthiopropionate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + 3-methylthiopropionyl-CoA | - |
? | |
ATP + 4-aminobutyrate + CoA | low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + 4-aminobutyryl-CoA | - |
? | |
ATP + acetate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + acetyl-CoA | - |
? | |
ATP + glycolate + CoA | low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + glycolyl-CoA | - |
? | |
ATP + isobutyrate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + isobutyryl-CoA | - |
? | |
ATP + isovalerate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + isovaleryl-CoA | - |
? | |
ATP + lactate + CoA | low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + lactoyl-CoA | - |
? | |
ATP + phenylacetate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + phenylacetyl-CoA | - |
? | |
ATP + thioglycolate + CoA | the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys | Thermococcus kodakarensis | ADP + phosphate + thioglycolyl-CoA | - |
? | |
additional information | activity with 2-(imidazol-4-yl)acetate is less than 5% of the activity with acetate | Thermococcus kodakarensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
ACSIIITk | - |
Thermococcus kodakarensis |
TK0944/TK0943 protein | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life of the TK2127/TK0943 protein: 380 min | Thermococcus kodakarensis |
90 | - |
half-life of the TK2127/TK0943 protein: 170 min | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
thioglycolate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 26.1/s | Thermococcus kodakarensis | |
additional information | - |
phenylacetate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 40.6/s | Thermococcus kodakarensis | |
additional information | - |
acetate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 49.3/s | Thermococcus kodakarensis | |
additional information | - |
Isobutyrate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 50.9/s | Thermococcus kodakarensis | |
53.1 | - |
CoA | pH 6.5, 55°C | Thermococcus kodakarensis | |
53.7 | - |
ATP | pH 6.5, 55°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
phenylacetate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 123/s*mM | Thermococcus kodakarensis | |
additional information | - |
acetate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 32.8/s*mM | Thermococcus kodakarensis | |
additional information | - |
Isobutyrate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 385/s*mM | Thermococcus kodakarensis | |
additional information | - |
thioglycolate | pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 81.0/s*mM | Thermococcus kodakarensis | |
965 | - |
ATP | pH 6.5, 55°C | Thermococcus kodakarensis | |
9490 | - |
CoA | pH 6.5, 55°C | Thermococcus kodakarensis |