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Literature summary for 6.2.1.B10 extracted from

  • Awano, T.; Wilming, A.; Tomita, H.; Yokooji, Y.; Fukui, T.; Imanaka, T.; Atomi, H.
    Characterization of two members among the five ADP-forming acyl coenzyme A (acyl-CoA) synthetases reveals the presence of a 2-(imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis (2014), J. Bacteriol., 196, 140-147.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.B10

Cloned(Commentary)

Cloned (Comment) Organism
coexpression with gene TK0943 in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 2-(imidazol-4-yl)acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.543 mM Thermococcus kodakarensis
0.03
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis
0.0625
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27148
-
 2 * 47645 + 2 * 27148, calculated from sequence Thermococcus kodakarensis
47645
-
 2 * 47645 + 2 * 27148, calculated from sequence Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-(imidazol-4-yl)acetate + CoA Thermococcus kodakarensis the enzyme is involved in catabolism of histidine ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JEW2 and Q5JIA9 Q5JEW2: alpha-subunit, Q5JIA9: beta-subunit
-

Purification (Commentary)

Purification (Comment) Organism
recombinant TK2127/TK0943 protein Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-(imidazol-4-yl)acetate + CoA the enzyme is involved in catabolism of histidine Thermococcus kodakarensis ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
-
 ?
ATP + 2-(imidazol-4-yl)acetate + CoA the TK2127/TK0943 protein exhibits a strict preference for 2-(imidazol-4-yl)acetate, the metabolite expected in the degradation of histidine Thermococcus kodakarensis ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 47645 + 2 * 27148, calculated from sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
2-(imidazol-4-yl)acetyl-CoA synthetase
-
 Thermococcus kodakarensis
ADP-forming 2-(imidazol-4-yl)acetyl-CoA synthetase
-
 Thermococcus kodakarensis
ICSTk
-
 Thermococcus kodakarensis
TK2127/TK0943 protein
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life of the TK2127/TK0943 protein: 140 min Thermococcus kodakarensis
90
-
 half-life of the TK2127/TK0943 protein: 120 min Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 2-(imidazol-4-yl)acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 37.8/s Thermococcus kodakarensis
37.9
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis
39.6
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 2-(imidazol-4-yl)acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 69.7/s* mM Thermococcus kodakarensis
633
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis
1260
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis