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Literature summary for 6.2.1.76 extracted from
- Malonyl-CoA synthetase, encoded by ACYL ACTIVATING ENZYME13, is essential for growth and development of Arabidopsis (2011), Plant Cell, 23, 2247-2262 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
| expression in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.529 | - |
malonate | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |  |
| 0.66 | - |
malonate | pH 7.5, temperature not specified in the publication | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60062 | - |
calculated from sequence | Arabidopsis thaliana |
| 64130 | - |
calculated from sequence | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + malonate + CoA | Arabidopsis thaliana | - |
AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | Homo sapiens | - |
AMP + diphosphate + malonyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8H151 | ecotype Columbia | - |
| Homo sapiens | Q4G176 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | high expression | Homo sapiens | - |
| bone | - |
Homo sapiens | - |
| bone marrow | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| connective tissue | - |
Homo sapiens | - |
| eye | - |
Homo sapiens | - |
| intestine | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| lymph | high expression | Homo sapiens | - |
| lymph node | high expression | Homo sapiens | - |
| mammary gland | - |
Homo sapiens | - |
| mouth | - |
Homo sapiens | - |
| nerve | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| thymus | high expression | Homo sapiens | - |
| tonsil | high expression | Homo sapiens | - |
| uterus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-methylmalonate + CoA | activity with 2-methylmalonate is 12% compared to the activity malonate | Arabidopsis thaliana | AMP + diphosphate + 2-methylmalonyl-CoA | - |
? | |
| ATP + malonate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | - |
Homo sapiens | AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | little or no activity against other dicarboxylic or fatty acids | Arabidopsis thaliana | AMP + diphosphate + malonyl-CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAE13 | - |
Arabidopsis thaliana |
| ACSF3 | - |
Homo sapiens |
| Acyl Activating Enzyme13 | - |
Arabidopsis thaliana |
| At3g16170 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | - |
Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | calculated from sequence | - |
5.95 |
| Homo sapiens | calculated from sequence | - |
8.37 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | aae13 null mutants grow poorly and accumulated malonic and succinic acids | Arabidopsis thaliana |
| physiological function | AAE13 is required to metabolize free malonate and prevent its accumulation to levels that are toxic to cell metabolism and plant growth | Arabidopsis thaliana |
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