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Literature summary for 6.2.1.76 extracted from

  • An, J.H.; Lee, G.Y.; Jung, J.W.; Lee, W.; Kim, Y.S.
    Identification of residues essential for a two-step reaction by malonyl-CoA synthetase from Rhizobium trifolii (1999), Biochem. J., 344, 159-166 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhizobium leguminosarum

Protein Variants

Protein Variants Comment Organism
H206L kcat value for malonyl-CoA formation is reduced 40fold Rhizobium leguminosarum
K170M kcat value for malonyl-CoA formation is reduced 2.2fold Rhizobium leguminosarum
R168G kcat value for malonyl-CoA formation is reduced 1.7fold. The mutant enzyme shows slightly higher methyl malonyl-CoA synthetase activity than the wild type Rhizobium leguminosarum
R168G/K170M kcat value for malonyl-CoA formation is reduced 6.1fold Rhizobium leguminosarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
 CoA pH 7.0, 30°C, mutant enzyme H206L, cosubstrate: malonate Rhizobium leguminosarum
0.0078
-
 CoA pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: methylmalonate Rhizobium leguminosarum
0.0081
-
 CoA pH 7.0, 30°C, wild-type enzyme, cosubstrate: methylmalonate Rhizobium leguminosarum
0.017
-
 CoA pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: methylmalonate Rhizobium leguminosarum
0.02
-
 ATP pH 7.0, 30°C, wild-type enzyme, cosubstrate: methylmalonate Rhizobium leguminosarum
0.046
-
 CoA pH 7.0, 30°C, wild-type enzyme, cosubstrate: malonate Rhizobium leguminosarum
0.052
-
 ATP pH 7.0, 30°C, wild-type enzyme, cosubstrate: malonate Rhizobium leguminosarum
0.054
-
 CoA pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: malonate Rhizobium leguminosarum
0.058
-
 ATP pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: methylmalonate Rhizobium leguminosarum
0.059
-
 ATP pH 7.0, 30°C, mutant enzyme H206L, cosubstrate: malonate Rhizobium leguminosarum
0.063
-
 ATP pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: malonate Rhizobium leguminosarum
0.072
-
 ATP pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: methylmalonate Rhizobium leguminosarum
0.074
-
 CoA pH 7.0, 30°C, mutant enzyme R168G/K170M, cosubstrate: malonate Rhizobium leguminosarum
0.076
-
 ATP pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: malonate Rhizobium leguminosarum
0.106
-
 ATP pH 7.0, 30°C, mutant enzyme R168G/K170M, cosubstrate: malonate Rhizobium leguminosarum
0.133
-
 CoA pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: malonate Rhizobium leguminosarum
0.208
-
 malonate pH 7.0, 30°C, wild-type enzyme Rhizobium leguminosarum
0.373
-
 methylmalonate pH 7.0, 30°C, wild-type enzyme Rhizobium leguminosarum
0.452
-
 malonate pH 7.0, 30°C, mutant enzyme R168G Rhizobium leguminosarum
2.24
-
 methylmalonate pH 7.0, 30°C, mutant enzyme R168G Rhizobium leguminosarum
2.6
-
 malonate pH 7.0, 30°C, mutant enzyme K170M Rhizobium leguminosarum
4.1
-
 methylmalonate pH 7.0, 30°C, mutant enzyme K170M Rhizobium leguminosarum
5.6
-
 malonate pH 7.0, 30°C, mutant enzyme R168G/K170M Rhizobium leguminosarum
8.7
-
 malonate pH 7.0, 30°C, mutant enzyme H206L Rhizobium leguminosarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 SDS-PAGE Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + malonate + CoA Rhizobium leguminosarum
-
 AMP + diphosphate + malonyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum Q9ZIP5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhizobium leguminosarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + malonate + CoA
-
 Rhizobium leguminosarum AMP + diphosphate + malonyl-CoA
-
 ?
ATP + malonate + CoA with activity with acetate, propionate, or succinate in place of malonate, or with GTP or CTP in place of ATP Rhizobium leguminosarum AMP + diphosphate + malonyl-CoA
-
 ?
ATP + methylmalonate + CoA the kcat/KM value for methylmalonate is 12.6fold lower than the kcat/Km value for malonate Rhizobium leguminosarum AMP + diphosphate + methylmalonyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
malonyl-CoA synthetase
-
 Rhizobium leguminosarum
matB
-
 Rhizobium leguminosarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 ATP pH 7.0, 30°C, mutant enzyme H206L, cosubstrate: malonate Rhizobium leguminosarum
0.53
-
 malonate pH 7.0, 30°C, mutant enzyme H206L Rhizobium leguminosarum
0.55
-
 CoA pH 7.0, 30°C, mutant enzyme H206L, cosubstrate: malonate Rhizobium leguminosarum
0.56
-
 CoA pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: methylmalonate Rhizobium leguminosarum
0.59
-
 methylmalonate pH 7.0, 30°C, mutant enzyme K170M Rhizobium leguminosarum
0.6
-
 ATP pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: methylmalonate Rhizobium leguminosarum
2.7
-
 methylmalonate pH 7.0, 30°C, wild-type enzyme Rhizobium leguminosarum
2.7
-
 ATP pH 7.0, 30°C, wild-type enzyme, cosubstrate: methylmalonate Rhizobium leguminosarum
2.9
-
 ATP pH 7.0, 30°C, mutant enzyme R168G/K170M, cosubstrate: malonate Rhizobium leguminosarum
3
-
 methylmalonate pH 7.0, 30°C, mutant enzyme R168G Rhizobium leguminosarum
3
-
 ATP pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: methylmalonate Rhizobium leguminosarum
3.1
-
 CoA pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: methylmalonate Rhizobium leguminosarum
3.2
-
 CoA pH 7.0, 30°C, mutant enzyme R168G/K170M, cosubstrate: malonate Rhizobium leguminosarum
3.3
-
 CoA pH 7.0, 30°C, wild-type enzyme, cosubstrate: methylmalonate Rhizobium leguminosarum
3.8
-
 malonate pH 7.0, 30°C, mutant enzyme R168G/K170M Rhizobium leguminosarum
9
-
 malonate pH 7.0, 30°C, mutant enzyme K170M Rhizobium leguminosarum
9
-
 ATP pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: malonate Rhizobium leguminosarum
9.2
-
 CoA pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: malonate Rhizobium leguminosarum
11
-
 malonate pH 7.0, 30°C, mutant enzyme R168G Rhizobium leguminosarum
12
-
 ATP pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: malonate Rhizobium leguminosarum
13
-
 CoA pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: malonate Rhizobium leguminosarum
19
-
 malonate pH 7.0, 30°C, wild-type enzyme Rhizobium leguminosarum
20
-
 ATP pH 7.0, 30°C, wild-type enzyme, cosubstrate: malonate Rhizobium leguminosarum
21
-
 CoA pH 7.0, 30°C, wild-type enzyme, cosubstrate: malonate Rhizobium leguminosarum

General Information

General Information Comment Organism
metabolism the enzyme plays a key role in malonate metabolism Rhizobium leguminosarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 malonate pH 7.0, 30°C, mutant enzyme H206L Rhizobium leguminosarum
0.14
-
 methylmalonate pH 7.0, 30°C, mutant enzyme K170M Rhizobium leguminosarum
0.68
-
 malonate pH 7.0, 30°C, mutant enzyme R168G/K170M Rhizobium leguminosarum
1.3
-
 methylmalonate pH 7.0, 30°C, mutant enzyme R168G Rhizobium leguminosarum
3.5
-
 malonate pH 7.0, 30°C, mutant enzyme K170M Rhizobium leguminosarum
6.4
-
 ATP pH 7.0, 30°C, mutant enzyme H206L, cosubstrate: malonate Rhizobium leguminosarum
7.2
-
 methylmalonate pH 7.0, 30°C, wild-type enzyme Rhizobium leguminosarum
8.3
-
 ATP pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: methylmalonate Rhizobium leguminosarum
24
-
 malonate pH 7.0, 30°C, mutant enzyme R168G Rhizobium leguminosarum
27
-
 ATP pH 7.0, 30°C, mutant enzyme R168G/K170M, cosubstrate: malonate Rhizobium leguminosarum
43
-
 CoA pH 7.0, 30°C, mutant enzyme R168G/K170M, cosubstrate: malonate Rhizobium leguminosarum
52
-
 ATP pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: methylmalonate Rhizobium leguminosarum
71
-
 CoA pH 7.0, 30°C, mutant enzyme H206L, cosubstrate: malonate Rhizobium leguminosarum
72
-
 CoA pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: methylmalonate Rhizobium leguminosarum
91
-
 malonate pH 7.0, 30°C, wild-type enzyme Rhizobium leguminosarum
98
-
 CoA pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: malonate Rhizobium leguminosarum
118
-
 ATP pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: malonate Rhizobium leguminosarum
135
-
 ATP pH 7.0, 30°C, wild-type enzyme, cosubstrate: methylmalonate Rhizobium leguminosarum
170
-
 CoA pH 7.0, 30°C, mutant enzyme K170M, cosubstrate: malonate Rhizobium leguminosarum
182
-
 CoA pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: methylmalonate Rhizobium leguminosarum
190
-
 ATP pH 7.0, 30°C, mutant enzyme R168G, cosubstrate: malonate Rhizobium leguminosarum
384
-
 ATP pH 7.0, 30°C, wild-type enzyme, cosubstrate: malonate Rhizobium leguminosarum
407
-
 CoA pH 7.0, 30°C, wild-type enzyme, cosubstrate: methylmalonate Rhizobium leguminosarum
456
-
 CoA pH 7.0, 30°C, wild-type enzyme, cosubstrate: malonate Rhizobium leguminosarum