Any feedback?
Literature summary for 6.2.1.76 extracted from
- Purification and properties of malonyl-CoA synthetase from Rhizobium japonicum (1991), Biochem. J., 273, 511-516 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive | Bradyrhizobium japonicum |  |
| AMP | competitive | Bradyrhizobium japonicum | |
| diethylpyrocarbonate | 2 mM, 7 min, almost complete loss of activity | Bradyrhizobium japonicum | |
| glycerol | when the glycerol content in the assay mixture was 1.24-3.24% (v/v), enzyme activity is highest. Increased or decreased glycerol content gradually decreases activity | Bradyrhizobium japonicum | |
| additional information | no inhibition: iodoacetamide or p-chloromercuriphenylsulphonate | Bradyrhizobium japonicum | |
| pyridoxal-5'-phosphate | 3 mM, 15 min, almost complete loss of activity | Bradyrhizobium japonicum | |
| succinate | non-competitive | Bradyrhizobium japonicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
ATP | pH 8.4, 30°C | Bradyrhizobium japonicum | |
| 0.087 | - |
CoA | pH 8.4, 30°C | Bradyrhizobium japonicum | |
| 0.2 | - |
malonate | pH 8.4, 30°C | Bradyrhizobium japonicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required, no difference in activity | Bradyrhizobium japonicum | |
| Mn2+ | Mn2+ or Mg2+ required, no difference in activity | Bradyrhizobium japonicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Bradyrhizobium japonicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + malonate + CoA | Bradyrhizobium japonicum | - |
AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | Bradyrhizobium japonicum USDA 110 | - |
AMP + diphosphate + malonyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bradyrhizobium japonicum | - |
- |
- |
| Bradyrhizobium japonicum USDA 110 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bradyrhizobium japonicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22.77 | - |
pH 8.4, 30°C | Bradyrhizobium japonicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme in 20 mM-phosphate buffer (pH 6.9), 15% glycerol, no detectable loss of activity after several months | Bradyrhizobium japonicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-methylmalonate + CoA | activity is 53.6% compared to the activity with malonate | Bradyrhizobium japonicum | AMP + diphosphate + 2-methyl-malonyl-CoA | - |
? | |
| ATP + 2-methylmalonate + CoA | activity is 53.6% compared to the activity with malonate | Bradyrhizobium japonicum USDA 110 | AMP + diphosphate + 2-methyl-malonyl-CoA | - |
? | |
| ATP + malonate + CoA | - |
Bradyrhizobium japonicum | AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | high substrate specificity on malonate and ATP | Bradyrhizobium japonicum | AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | - |
Bradyrhizobium japonicum USDA 110 | AMP + diphosphate + malonyl-CoA | - |
? | |
| ATP + malonate + CoA | high substrate specificity on malonate and ATP | Bradyrhizobium japonicum USDA 110 | AMP + diphosphate + malonyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Bradyrhizobium japonicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malonyl-CoA synthetase | - |
Bradyrhizobium japonicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme loses 80% of activity within 30 min. When ATP and malonate are added to the enzyme solution, activity loss is reduced down to 50% | Bradyrhizobium japonicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Bradyrhizobium japonicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
AMP | pH 8.4, 30°C | Bradyrhizobium japonicum | |
| 0.25 | - |
ADP | pH 8.4, 30°C | Bradyrhizobium japonicum | |
| 47.3 | - |
succinate | pH 8.4, 30°C | Bradyrhizobium japonicum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bradyrhizobium japonicum | isoelectric focusing | - |
7.3 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is not inducible | Bradyrhizobium japonicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
