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Literature summary for 6.2.1.74 extracted from
- The loading and initial elongation modules of rifamycin synthetase collaborate to produce mixed aryl ketide products (2002), Biochemistry, 41, 5313-5324 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | expression of the N-terminal 260 kDa loading and initial elongation modules of rifamycin synthetase | Amycolatopsis mediterranei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
benzoate | pH 7.2, 30°C | Amycolatopsis mediterranei |  |
| 1.1 | - |
3-hydroxybenzoate | pH 7.2, 30°C | Amycolatopsis mediterranei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amycolatopsis mediterranei | O54666 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phospholipoprotein | activity requires phosphopantetheinylation of the thiolation domain of RifA | Amycolatopsis mediterranei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-amino-5-hydroxybenzoate + a holo-[acyl-carrier protein] | - |
Amycolatopsis mediterranei | 3-amino-5-hydroxybenzoyl-[acyl-carrier protein] + AMP + diphosphate | - |
? | |
| ATP + 3-hydroxybenzoate + a holo-[acyl-carrier protein] | - |
Amycolatopsis mediterranei | 3-hydroxybenzoyl-[acyl-carrier protein] + AMP + diphosphate | - |
? | |
| ATP + benzoate + a holo-[acyl-carrier protein] | - |
Amycolatopsis mediterranei | benzoyl-[acyl-carrier protein] + AMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rifA | - |
Amycolatopsis mediterranei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
benzoate | pH 7.2, 30°C | Amycolatopsis mediterranei | |
| 0.008 | - |
3-hydroxybenzoate | pH 7.2, 30°C | Amycolatopsis mediterranei | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00167 | - |
benzoate | pH 7.2, 30°C | Amycolatopsis mediterranei | |
| 0.00767 | - |
3-hydroxybenzoate | pH 7.2, 30°C | Amycolatopsis mediterranei | |
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Release 2023.1 (January 2023)
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