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Literature summary for 6.2.1.71 extracted from

  • Sikora, A.; Wilson, D.; Aldrich, C.; Blanchard, J.
    Kinetic and inhibition studies of dihydroxybenzoate-AMP ligase from Escherichia coli (2010), Biochemistry, 49, 3648-3657 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.71

Inhibitors

Inhibitors Comment Organism Structure
5'-O-[N-(2,3-dihydroxybenzoyl)sulfamoyl]adenosine acts as slow-onset tight-binding inhibitor Escherichia coli
5'-O-[N-(salicyl)sulfamoyl]adenosine i.e.Sal-AMS, acts as slow-onset tight-binding inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 2,3-Dihydroxybenzoate pH 7.8, 25°C Escherichia coli
0.0029
-
 [aryl-carrier protein EntB] pH 7.8, 25°C Escherichia coli
0.07
-
 salicylate pH 7.8, 25°C Escherichia coli
0.07
-
 3-hydroxybenzoate pH 7.8, 25°C Escherichia coli
0.43
-
 ATP pH 7.8, 25°C Escherichia coli
3.1
-
 4-Aminosalicylate pH 7.8, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P10378
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2,3-dihydroxybenzoate + [aryl-carrier protein EntB]
-
 Escherichia coli AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB]
-
 ?
ATP + 3-hydroxybenzoate + [aryl-carrier protein EntB]
-
 Escherichia coli AMP + diphosphate + 3-hydroxybenzoyl-[aryl-carrier protein EntB]
-
 ?
ATP + 4-aminosalicylate + [aryl-carrier protein EntB]
-
 Escherichia coli AMP + diphosphate + 4-aminosalicyl-[aryl-carrier protein EntB]
-
 ?
ATP + salicylate + [aryl-carrier protein EntB]
-
 Escherichia coli AMP + diphosphate + salicyl-[aryl-carrier protein EntB]
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 3-hydroxybenzoate pH 7.8, 25°C Escherichia coli
0.8
-
 salicylate pH 7.8, 25°C Escherichia coli
2.8
-
 ATP pH 7.8, 25°C Escherichia coli
2.8
-
 2,3-Dihydroxybenzoate pH 7.8, 25°C Escherichia coli
2.8
-
 [aryl-carrier protein EntB] pH 7.8, 25°C Escherichia coli
4.4
-
 4-Aminosalicylate pH 7.8, 25°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000009
-
 5'-O-[N-(salicyl)sulfamoyl]adenosine pH 7.8, 25°C Escherichia coli
0.0000038
-
 5'-O-[N-(2,3-dihydroxybenzoyl)sulfamoyl]adenosine pH 7.8, 25°C Escherichia coli

General Information

General Information Comment Organism
metabolism EntE proceeds via a Bi Uni Uni Bi ping-pong kinetic mechanism. During the first half-reaction (adenylation), dihydroxybenzoate binds first to the free enzyme, followed by ATP and the release of diphosphate to form the adenylate intermediate. During the second half-reaction (ligation), phosphopantetheinylated EntB binds to the enzyme followed by the release of products, AMP and arylated EntB Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.6
-
 3-hydroxybenzoate pH 7.8, 25°C Escherichia coli
11
-
 salicylate pH 7.8, 25°C Escherichia coli
15
-
 4-Aminosalicylate pH 7.8, 25°C Escherichia coli
390
-
 ATP pH 7.8, 25°C Escherichia coli
880
-
 2,3-Dihydroxybenzoate pH 7.8, 25°C Escherichia coli
980
-
 [aryl-carrier protein EntB] pH 7.8, 25°C Escherichia coli