Literature summary for 6.2.1.71 extracted from

Gehring, A.M.; Bradley, K.A.; Walsh, C.T.
Enterobactin biosynthesis in Escherichia coli isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate (1997), Biochemistry, 36, 8495-8503 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0004	-	holo-[aryl-carrier protein EntB]	pH 7.5, 37°C	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2,3-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein EntB]	Escherichia coli	-	AMP + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB]	-	?
ATP + 2,3-dihydroxybenzoate	Escherichia coli	-	diphosphate + (2,3-dihydroxybenzoyl)adenylate	-	?
ATP + 2,3-dihydroxybenzoate + holo-[aryl-carrier protein EntB]	Escherichia coli	-	AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P10378	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2,3-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein EntB]	-	Escherichia coli	AMP + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB]	-	?
ATP + 2,3-dihydroxybenzoate	-	Escherichia coli	diphosphate + (2,3-dihydroxybenzoyl)adenylate	-	?
ATP + 2,3-dihydroxybenzoate + holo-[aryl-carrier protein EntB]	-	Escherichia coli	AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB]	-	?
ATP + salicylate + holo-[aryl-carrier protein EntB]	-	Escherichia coli	AMP + diphosphate + salicyl-[aryl-carrier protein EntB]	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.67	-	holo-[aryl-carrier protein EntB]	pH 7.5, 37°C	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	the holo-EntB ArCP domain is acylated with 2,3-dihydroxybenzoate in a reaction in which EntE catalyzes the activation of 2,3-dihydroxybenzoate by adenylation as well as the transfer of 2,3-dihydroxybenzoate to the phosphopantetheine cofactor of holo-EntB	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4167	-	holo-[aryl-carrier protein EntB]	pH 7.5, 37°C	Escherichia coli

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Release 2023.1 (January 2023)