Cloned (Comment) | Organism |
---|---|
the biosynthetic gene cluster for SFM-A is cloned and localized to a 62-kb contiguous DNA region. Sequence analysis reveals 30 genes that constitute the SFM-A gene cluster, encoding an unusual nonribosomal peptide synthetase (NRPS) system and tailoring enzymes and regulatory and resistance proteins. Three NRPS genes, sfmA, sfmB and sfmC, are identified within the sfm cluster. Cloning of gene sfmB, part of the SFM-A gene cluster, DNA and amino acid sequence determination and analysis, genetic organization, recombinant expression in Pseudomonas fluorescens | Streptomyces lavendulae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces lavendulae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(glycyl)adenylate + holo-[glycyl-carrier protein] | Streptomyces lavendulae | - |
AMP + glycyl-[glycyl-carrier protein] | - |
ir | |
(glycyl)adenylate + holo-[glycyl-carrier protein] | Streptomyces lavendulae NRRL 11002 | - |
AMP + glycyl-[glycyl-carrier protein] | - |
ir | |
ATP + glycine | Streptomyces lavendulae | - |
diphosphate + (glycyl)adenylate | - |
ir | |
ATP + glycine | Streptomyces lavendulae NRRL 11002 | - |
diphosphate + (glycyl)adenylate | - |
ir | |
ATP + glycine + holo-[glycyl-carrier protein] | Streptomyces lavendulae | - |
AMP + diphosphate + glycyl-[glycyl-carrier protein] | - |
ir | |
ATP + glycine + holo-[glycyl-carrier protein] | Streptomyces lavendulae NRRL 11002 | - |
AMP + diphosphate + glycyl-[glycyl-carrier protein] | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lavendulae | B0CN26 | - |
- |
Streptomyces lavendulae NRRL 11002 | B0CN26 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(glycyl)adenylate + holo-[glycyl-carrier protein] | - |
Streptomyces lavendulae | AMP + glycyl-[glycyl-carrier protein] | - |
ir | |
(glycyl)adenylate + holo-[glycyl-carrier protein] | - |
Streptomyces lavendulae NRRL 11002 | AMP + glycyl-[glycyl-carrier protein] | - |
ir | |
ATP + glycine | - |
Streptomyces lavendulae | diphosphate + (glycyl)adenylate | - |
ir | |
ATP + glycine | - |
Streptomyces lavendulae NRRL 11002 | diphosphate + (glycyl)adenylate | - |
ir | |
ATP + glycine + holo-[glycyl-carrier protein] | - |
Streptomyces lavendulae | AMP + diphosphate + glycyl-[glycyl-carrier protein] | - |
ir | |
ATP + glycine + holo-[glycyl-carrier protein] | - |
Streptomyces lavendulae NRRL 11002 | AMP + diphosphate + glycyl-[glycyl-carrier protein] | - |
ir | |
additional information | recombinant SfmB (C2-A2-PCP2) shows exclusive activities with Gly, determination of substrate specificities of SfmB by utilizing amino acid-dependent ATP-diphosphate exchange assay | Streptomyces lavendulae | ? | - |
- |
|
additional information | recombinant SfmB (C2-A2-PCP2) shows exclusive activities with Gly, determination of substrate specificities of SfmB by utilizing amino acid-dependent ATP-diphosphate exchange assay | Streptomyces lavendulae NRRL 11002 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
nonribosomal peptide synthetase | - |
Streptomyces lavendulae |
sfmB | - |
Streptomyces lavendulae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces lavendulae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces lavendulae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4'-phosphopantetheine | - |
Streptomyces lavendulae | |
ATP | - |
Streptomyces lavendulae |
General Information | Comment | Organism |
---|---|---|
malfunction | the sfmB mutant strain TL2003 completely loses its ability to produce SFM-A, which is restored by expressing sfmB in trans, confirming the essential role of this NRPS system for SFM-A biosynthesis | Streptomyces lavendulae |
metabolism | proposed biosynthetic pathway for saframycin A involving genes sfmA, sfmB, and sfmC, overview | Streptomyces lavendulae |
additional information | the biosynthetic gene cluster for SFM-A is cloned and localized to a 62-kb contiguous DNA region. Sequence analysis revealed 30 genes that constitute the SFM-A gene cluster, encoding an unusual nonribosomal peptide synthetase (NRPS) system and tailoring enzymes and regulatory and resistance proteins. The results of substrate prediction and in vitro characterization of the adenylation specificities of this NRPS system support the hypothesis that the last module acts in an iterative manner to form a tetrapeptidyl intermediate and that the co-linearity rule does not apply | Streptomyces lavendulae |
physiological function | the enzyme is involved in the synthesis of saframycin A (SFM-A) by Streptomyces lavendulae strain NRRL 11002. The compound belongs to the tetrahydroisoquinoline family of antibiotics. The backbone of SFM-A is derived from one Ala, one Gly, and two Tyr residues, suggesting that it is of tetrapeptide origin. SfmA, SfmB, and SfmC constitute an NRPS system | Streptomyces lavendulae |