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Literature summary for 6.2.1.66 extracted from

  • Li, L.; Deng, W.; Song, J.; Ding, W.; Zhao, Q.; Peng, C.; Song, W.; Tang, G.; Liu, W.
    Characterization of the saframycin A gene cluster from Streptomyces lavendulae NRRL 11002 revealing a nonribosomal peptide synthetase system for assembling the unusual tetrapeptidyl skeleton in an iterative manner (2008), J. Bacteriol., 190, 251-263 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the biosynthetic gene cluster for SFM-A is cloned and localized to a 62-kb contiguous DNA region. Sequence analysis reveals 30 genes that constitute the SFM-A gene cluster, encoding an unusual nonribosomal peptide synthetase (NRPS) system and tailoring enzymes and regulatory and resistance proteins. Three NRPS genes, sfmA, sfmB and sfmC, are identified within the sfm cluster. Cloning of gene sfmB, part of the SFM-A gene cluster, DNA and amino acid sequence determination and analysis, genetic organization, recombinant expression in Pseudomonas fluorescens Streptomyces lavendulae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(glycyl)adenylate + holo-[glycyl-carrier protein] Streptomyces lavendulae
-
AMP + glycyl-[glycyl-carrier protein]
-
ir
(glycyl)adenylate + holo-[glycyl-carrier protein] Streptomyces lavendulae NRRL 11002
-
AMP + glycyl-[glycyl-carrier protein]
-
ir
ATP + glycine Streptomyces lavendulae
-
diphosphate + (glycyl)adenylate
-
ir
ATP + glycine Streptomyces lavendulae NRRL 11002
-
diphosphate + (glycyl)adenylate
-
ir
ATP + glycine + holo-[glycyl-carrier protein] Streptomyces lavendulae
-
AMP + diphosphate + glycyl-[glycyl-carrier protein]
-
ir
ATP + glycine + holo-[glycyl-carrier protein] Streptomyces lavendulae NRRL 11002
-
AMP + diphosphate + glycyl-[glycyl-carrier protein]
-
ir

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae B0CN26
-
-
Streptomyces lavendulae NRRL 11002 B0CN26
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(glycyl)adenylate + holo-[glycyl-carrier protein]
-
Streptomyces lavendulae AMP + glycyl-[glycyl-carrier protein]
-
ir
(glycyl)adenylate + holo-[glycyl-carrier protein]
-
Streptomyces lavendulae NRRL 11002 AMP + glycyl-[glycyl-carrier protein]
-
ir
ATP + glycine
-
Streptomyces lavendulae diphosphate + (glycyl)adenylate
-
ir
ATP + glycine
-
Streptomyces lavendulae NRRL 11002 diphosphate + (glycyl)adenylate
-
ir
ATP + glycine + holo-[glycyl-carrier protein]
-
Streptomyces lavendulae AMP + diphosphate + glycyl-[glycyl-carrier protein]
-
ir
ATP + glycine + holo-[glycyl-carrier protein]
-
Streptomyces lavendulae NRRL 11002 AMP + diphosphate + glycyl-[glycyl-carrier protein]
-
ir
additional information recombinant SfmB (C2-A2-PCP2) shows exclusive activities with Gly, determination of substrate specificities of SfmB by utilizing amino acid-dependent ATP-diphosphate exchange assay Streptomyces lavendulae ?
-
-
additional information recombinant SfmB (C2-A2-PCP2) shows exclusive activities with Gly, determination of substrate specificities of SfmB by utilizing amino acid-dependent ATP-diphosphate exchange assay Streptomyces lavendulae NRRL 11002 ?
-
-

Synonyms

Synonyms Comment Organism
nonribosomal peptide synthetase
-
Streptomyces lavendulae
sfmB
-
Streptomyces lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptomyces lavendulae

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine
-
Streptomyces lavendulae
ATP
-
Streptomyces lavendulae

General Information

General Information Comment Organism
malfunction the sfmB mutant strain TL2003 completely loses its ability to produce SFM-A, which is restored by expressing sfmB in trans, confirming the essential role of this NRPS system for SFM-A biosynthesis Streptomyces lavendulae
metabolism proposed biosynthetic pathway for saframycin A involving genes sfmA, sfmB, and sfmC, overview Streptomyces lavendulae
additional information the biosynthetic gene cluster for SFM-A is cloned and localized to a 62-kb contiguous DNA region. Sequence analysis revealed 30 genes that constitute the SFM-A gene cluster, encoding an unusual nonribosomal peptide synthetase (NRPS) system and tailoring enzymes and regulatory and resistance proteins. The results of substrate prediction and in vitro characterization of the adenylation specificities of this NRPS system support the hypothesis that the last module acts in an iterative manner to form a tetrapeptidyl intermediate and that the co-linearity rule does not apply Streptomyces lavendulae
physiological function the enzyme is involved in the synthesis of saframycin A (SFM-A) by Streptomyces lavendulae strain NRRL 11002. The compound belongs to the tetrahydroisoquinoline family of antibiotics. The backbone of SFM-A is derived from one Ala, one Gly, and two Tyr residues, suggesting that it is of tetrapeptide origin. SfmA, SfmB, and SfmC constitute an NRPS system Streptomyces lavendulae