Literature summary for 6.2.1.64 extracted from

Ohki, Y.; Funatsu, N.; Konishi, N.; Chiba, T.
The mechanism of poly-NEDD8 chain formation in vitro (2009), Biochem. Biophys. Res. Commun., 381, 443-447 .

Search for more literature for 6.2.1.64

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	Mus musculus	-	AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni Sepharose 6 column chromatography and Talon affinity resin column chromatography, and glutathione Sepharose 4 column chromatography	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine	-	Mus musculus	AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine	-	?

Subunits

Subunits	Comment	Organism
?	x * about 50000, Uba3, SDS-PAGE	Mus musculus
?	x * about 80000, APPBP1, SDS-PAGE	Mus musculus

Synonyms

Synonyms	Comment	Organism
APPBP1	-	Mus musculus
NEDD8 activating E1-like enzyme	-	Mus musculus
Uba3	-	Mus musculus

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Release 2023.1 (January 2023)