Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.56 extracted from

  • Koenneke, M.; Schubert, D.; Brown, P.; Huegler, M.; Standfest, S.; Schwander, T.; Schada Von Borzyskowski, L.; Erb, T.; Stahl, D.; Berg, I.
    Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO2 fixation (2014), Proc. Natl. Acad. Sci. USA, 111, 8239-8244 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.56

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nitrosopumilus maritimus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 CoA pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus
0.22
-
 ATP pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus
0.37
-
 4-hydroxybutanoate pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus
5
-
 Butanoate pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, required Nitrosopumilus maritimus
Mn2+ or Mg2+, required Nitrosopumilus maritimus
additional information not activating: Ni2+, Co2+, Ca2+ Nitrosopumilus maritimus

Organism

Organism UniProt Comment Textmining
Nitrosopumilus maritimus A9A1Y1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 cell extract, pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-hydroxybutanoate + CoA
-
 Nitrosopumilus maritimus ADP + phosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + butanoate + CoA
-
 Nitrosopumilus maritimus ADP + phosphate + butanoyl-CoA
-
 ?
additional information very poor substrates: acetate, propionate Nitrosopumilus maritimus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76500, calculated from sequence Nitrosopumilus maritimus

Synonyms

Synonyms Comment Organism
4-hydroxybutyryl-CoA synthetase
-
 Nitrosopumilus maritimus
Nmar_0206
-
 Nitrosopumilus maritimus

General Information

General Information Comment Organism
physiological function the enzyme participates in a variant of the 3-hydroxypropanoate/4-hydroxybutanate CO2 fixation cycle Nitrosopumilus maritimus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 Butanoate pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus
4.88
-
 4-hydroxybutanoate pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus
9.96
-
 ATP pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus
11.28
-
 CoA pH not specified in the publication, temperature not specified in the publication Nitrosopumilus maritimus