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Literature summary for 6.2.1.54 extracted from
- Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP (2009), J. Mol. Biol., 388, 345-355.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the pET28a vector is used | Bacillus cereus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) is determined in complex with ATP to 1.9 A resolution | Bacillus cereus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D383N | mutant, an invariant amino residue in the ATP-binding pocket is choosen for mutagenesis studies | Bacillus cereus |
| E298Q | mutant, an invariant amino residue in the ATP-binding pocket is choosen for mutagenesis studies | Bacillus cereus |
| R397Q | mutant, an invariant amino residue in the ATP-binding pocket is choosen for mutagenesis studies | Bacillus cereus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
ATP | wild-type | Bacillus cereus |  |
| 0.06 | - |
ATP | mutant R397Q | Bacillus cereus | |
| 0.08 | - |
ATP | mutant E298Q | Bacillus cereus | |
| 0.75 | - |
ATP | mutant D383N | Bacillus cereus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-alanine + poly(ribitol phosphate) | Bacillus cereus | - |
AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | Q81G39 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-alanine + poly(ribitol phosphate) | - |
Bacillus cereus | AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-alanyl carrier protein ligase | - |
Bacillus cereus |
| DltA | - |
Bacillus cereus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
ATP | mutant R397Q | Bacillus cereus | |
| 0.016 | - |
ATP | mutant E298Q | Bacillus cereus | |
| 0.097 | - |
ATP | mutant D383N | Bacillus cereus | |
| 0.215 | - |
ATP | wild-type | Bacillus cereus | |
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