Literature summary for 6.2.1.54 extracted from

May, J.J.; Finking, R.; Wiegeshoff, F.; Weber, T.T.; Bandur, N.; Koert, U.; Marahiel, M.A.
Inhibition of the D-alanine:D-alanyl carrier protein ligase from Bacillus subtilis increases the bacterium's susceptibility to antibiotics that target the cell wall (2005), FEBS J., 272, 2993-3003.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of DltA and DltC in Escherichia coli	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-O-[N-(D-alanyl)-sulfamoyl]adenosine	inhibits D-Ala adenylylation by DltA in vitro	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-alanine + poly(ribitol phosphate)	Bacillus subtilis	the biosynthesis of D-alanyl-lipoteichoic acid requires four proteins that are encoded by the dlt operon. The synthesis starts with the selection of the D-Ala by the 57000 Da D-Ala-D-Ala carrier protein ligase (DltA). Following activation by DltA, D-Ala is transferred to the 10000 Da D-alanyl carrier protein DltC which can donate D-Ala to lipoteichoic acids with the help of DltB and DltD to mediate the surface charge of the bacterium	AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-alanine + poly(ribitol phosphate)	the biosynthesis of D-alanyl-lipoteichoic acid requires four proteins that are encoded by the dlt operon. The synthesis starts with the selection of the D-Ala by the 57000 Da D-Ala-D-Ala carrier protein ligase (DltA). Following activation by DltA, D-Ala is transferred to the 10000 Da D-alanyl carrier protein DltC which can donate D-Ala to lipoteichoic acids with the help of DltB and DltD to mediate the surface charge of the bacterium	Bacillus subtilis	AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)	-	?

Synonyms

Synonyms	Comment	Organism
D-alanine:D-alanyl carrier protein ligase	-	Bacillus subtilis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000232	-	5'-O-[N-(D-alanyl)-sulfamoyl]adenosine	-	Bacillus subtilis

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Release 2023.1 (January 2023)