Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
expression in Escherichia coli | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
L-proline | pH 7.5, 25°C | Pseudomonas fluorescens | |
1.54 | - |
L-proline | pH 7.5, 25°C | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | Pseudomonas fluorescens | - |
AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | Streptomyces coelicolor | - |
AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | Streptomyces coelicolor A(3)2 | - |
AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | Pseudomonas fluorescens ATCC BAA-4770 | - |
AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline | Pseudomonas fluorescens | - |
diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline | Streptomyces coelicolor | - |
diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline | Streptomyces coelicolor A(3)2 | - |
diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline | Pseudomonas fluorescens ATCC BAA-4770 | - |
diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | Pseudomonas fluorescens | - |
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | Streptomyces coelicolor | - |
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | Streptomyces coelicolor A(3)2 | - |
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | Pseudomonas fluorescens ATCC BAA-4770 | - |
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
additional information | Pseudomonas fluorescens | very poor substrates: D-proline, glycine, no substrate: L-serine | ? | - |
? | |
additional information | Streptomyces coelicolor | very poor substrates: D-proline, glycine, no substrate: L-serine | ? | - |
? | |
additional information | Streptomyces coelicolor A(3)2 | very poor substrates: D-proline, glycine, no substrate: L-serine | ? | - |
? | |
additional information | Pseudomonas fluorescens ATCC BAA-4770 | very poor substrates: D-proline, glycine, no substrate: L-serine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | Q4KCY5 | - |
- |
Pseudomonas fluorescens ATCC BAA-4770 | Q4KCY5 | - |
- |
Streptomyces coelicolor | Q4KCY5 | - |
- |
Streptomyces coelicolor A(3)2 | Q4KCY5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas fluorescens |
recombinant protein | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | - |
Pseudomonas fluorescens | AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | - |
Streptomyces coelicolor | AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | - |
Streptomyces coelicolor A(3)2 | AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] | - |
Pseudomonas fluorescens ATCC BAA-4770 | AMP + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline | - |
Pseudomonas fluorescens | diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline | - |
Streptomyces coelicolor | diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline | - |
Streptomyces coelicolor A(3)2 | diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline | - |
Pseudomonas fluorescens ATCC BAA-4770 | diphosphate + (L-prolyl)adenylate | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein PltL] | - |
Pseudomonas fluorescens | AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein PltL] | - |
Streptomyces coelicolor | AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | - |
Pseudomonas fluorescens | AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | - |
Streptomyces coelicolor | AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | - |
Streptomyces coelicolor A(3)2 | AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
ATP + L-proline + holo-[L-prolyl-carrier protein] | - |
Pseudomonas fluorescens ATCC BAA-4770 | AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine | Pseudomonas fluorescens | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine | Streptomyces coelicolor | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate | Pseudomonas fluorescens | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate | Streptomyces coelicolor | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine | Streptomyces coelicolor A(3)2 | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate | Streptomyces coelicolor A(3)2 | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine | Pseudomonas fluorescens ATCC BAA-4770 | ? | - |
? | |
additional information | very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate | Pseudomonas fluorescens ATCC BAA-4770 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-prolyl-AMP ligase | - |
Pseudomonas fluorescens |
L-prolyl-AMP ligase | - |
Streptomyces coelicolor |
pltF | - |
Pseudomonas fluorescens |
SC3F7.11 | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
25 | - |
assay at | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
L-proline | pH 7.5, 25°C | Streptomyces coelicolor | |
5.5 | - |
L-proline | pH 7.5, 25°C | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas fluorescens |
7.5 | - |
assay at | Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein | Pseudomonas fluorescens |
physiological function | involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein | Streptomyces coelicolor |