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Literature summary for 6.2.1.53 extracted from

  • Thomas, M.; Burkart, M.; Walsh, C.
    Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis (2002), Chem. Biol., 9, 171-184 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens
expression in Escherichia coli Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
L-proline pH 7.5, 25°C Pseudomonas fluorescens
1.54
-
L-proline pH 7.5, 25°C Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens
-
AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Streptomyces coelicolor
-
AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Streptomyces coelicolor A(3)2
-
AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens ATCC BAA-4770
-
AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline Pseudomonas fluorescens
-
diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline Streptomyces coelicolor
-
diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline Streptomyces coelicolor A(3)2
-
diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline Pseudomonas fluorescens ATCC BAA-4770
-
diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens
-
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein] Streptomyces coelicolor
-
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein] Streptomyces coelicolor A(3)2
-
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein] Pseudomonas fluorescens ATCC BAA-4770
-
AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
additional information Pseudomonas fluorescens very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
?
additional information Streptomyces coelicolor very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
?
additional information Streptomyces coelicolor A(3)2 very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
?
additional information Pseudomonas fluorescens ATCC BAA-4770 very poor substrates: D-proline, glycine, no substrate: L-serine ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q4KCY5
-
-
Pseudomonas fluorescens ATCC BAA-4770 Q4KCY5
-
-
Streptomyces coelicolor Q4KCY5
-
-
Streptomyces coelicolor A(3)2 Q4KCY5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas fluorescens
recombinant protein Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
Pseudomonas fluorescens AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
Streptomyces coelicolor AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
Streptomyces coelicolor A(3)2 AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
(L-prolyl)adenylate + holo-[L-prolyl-carrier protein]
-
Pseudomonas fluorescens ATCC BAA-4770 AMP + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline
-
Pseudomonas fluorescens diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline
-
Streptomyces coelicolor diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline
-
Streptomyces coelicolor A(3)2 diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline
-
Pseudomonas fluorescens ATCC BAA-4770 diphosphate + (L-prolyl)adenylate
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
-
Pseudomonas fluorescens AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein PltL]
-
Streptomyces coelicolor AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein PltL]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
-
Pseudomonas fluorescens AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
-
Streptomyces coelicolor AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
-
Streptomyces coelicolor A(3)2 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
ATP + L-proline + holo-[L-prolyl-carrier protein]
-
Pseudomonas fluorescens ATCC BAA-4770 AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein]
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine Pseudomonas fluorescens ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine Streptomyces coelicolor ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Pseudomonas fluorescens ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Streptomyces coelicolor ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine Streptomyces coelicolor A(3)2 ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Streptomyces coelicolor A(3)2 ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine Pseudomonas fluorescens ATCC BAA-4770 ?
-
?
additional information very poor substrates: D-proline, glycine, no substrate: L-serine, pyrrolyl-2-carboxylate Pseudomonas fluorescens ATCC BAA-4770 ?
-
?

Synonyms

Synonyms Comment Organism
L-prolyl-AMP ligase
-
Pseudomonas fluorescens
L-prolyl-AMP ligase
-
Streptomyces coelicolor
pltF
-
Pseudomonas fluorescens
SC3F7.11
-
Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas fluorescens
25
-
assay at Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
L-proline pH 7.5, 25°C Streptomyces coelicolor
5.5
-
L-proline pH 7.5, 25°C Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas fluorescens
7.5
-
assay at Streptomyces coelicolor

General Information

General Information Comment Organism
physiological function involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein Pseudomonas fluorescens
physiological function involved in the undecylprodigiosin and pyoluteorin biosynthetic pathways, enzyme catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein Streptomyces coelicolor