Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Zophobas atratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
ATP | at pH 8.5, temperature not specified in the publication | Zophobas atratus | |
0.8 | - |
L-firefly luciferin | at pH 8.5, temperature not specified in the publication | Zophobas atratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-firefly luciferin + CoA | Zophobas atratus | - |
AMP + diphosphate + L-firefly luciferyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zophobas atratus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MiniQ anion exchange column chromatography, and Superdex 200 gel filtration | Zophobas atratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-firefly luciferin + CoA | - |
Zophobas atratus | AMP + diphosphate + L-firefly luciferyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Zophobas atratus |
Synonyms | Comment | Organism |
---|---|---|
protoluciferase | - |
Zophobas atratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
ATP | at pH 8.5, temperature not specified in the publication | Zophobas atratus | |
0.0015 | - |
L-firefly luciferin | at pH 8.5, temperature not specified in the publication | Zophobas atratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Zophobas atratus |