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Literature summary for 6.2.1.5 extracted from

  • Wider, E.A.; Tigier, H.A.
    Porphyrin biosynthesis in soybean callus tissue system. VIII. Isolation, purification and general properties of succinyl CoA synthetase (1971), Enzymologia, 41, 217-231.
    View publication on PubMed
Search for more literature for 6.2.1.5

Activating Compound

Activating Compound Comment Organism Structure
8-hydroxyquinoline 5 mM, enhances activity Glycine max
glutathione activates Glycine max
NaCN 10 mM , enhances activity Glycine max

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Glycine max
acetate
-
 Glycine max
ADP
-
 Glycine max
AMP
-
 Glycine max
ATP above 10 mM Glycine max
Butyrate
-
 Glycine max
Ca2+ 10 mM, 75% inhibition Glycine max
Co2+ up to 1 mM complete inhibition Glycine max
Cu2+ up to 10 mM complete inhibition Glycine max
cystine
-
 Glycine max
EDTA
-
 Glycine max
GSSG
-
 Glycine max
hemin
-
 Glycine max
Hg2+ up to 1 mM complete inhibition Glycine max
iodoacetamide
-
 Glycine max
Iodosobenzoate
-
 Glycine max
malonate
-
 Glycine max
NEM
-
 Glycine max
Pb2+ up to 10 mM complete inhibibition Glycine max
PCMB
-
 Glycine max
propionate
-
 Glycine max
protoporphyrin IX
-
 Glycine max
sodium arsenite
-
 Glycine max
succinate above 0.2 M Glycine max
Zn2+ up to 1 mM complete inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
 ATP soluble enzyme Glycine max
14
-
 succinate soluble enzyme Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 1.5 mM, soluble enzyme Glycine max
Mg2+ absolute requirement for either Mg2+ or Mn2+, optimal concentration of Mg2+ is 5 mM Glycine max
Mn2+ absolute requirement for either Mg2+ or Mn2+, Km: 3.5 mM Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max enzyme is involved in the initial step of porphyrin biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
 soybean
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max

Storage Stability

Storage Stability Organism
stable for several months in the frozen state Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + succinate + CoA specific for ATP Glycine max ADP + phosphate + succinyl-CoA
-
 ?
ATP + succinate + CoA specific for succinate Glycine max ADP + phosphate + succinyl-CoA
-
 ?
additional information enzyme is involved in the initial step of porphyrin biosynthesis Glycine max ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9 8.3
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.1 9 pH 7.1: about 75% of maximal activity, pH 9.0: about 70% of maximal activity Glycine max