Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 5 mM, enhances activity | Glycine max | |
glutathione | activates | Glycine max | |
NaCN | 10 mM , enhances activity | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Glycine max | |
acetate | - |
Glycine max | |
ADP | - |
Glycine max | |
AMP | - |
Glycine max | |
ATP | above 10 mM | Glycine max | |
Butyrate | - |
Glycine max | |
Ca2+ | 10 mM, 75% inhibition | Glycine max | |
Co2+ | up to 1 mM complete inhibition | Glycine max | |
Cu2+ | up to 10 mM complete inhibition | Glycine max | |
cystine | - |
Glycine max | |
EDTA | - |
Glycine max | |
GSSG | - |
Glycine max | |
hemin | - |
Glycine max | |
Hg2+ | up to 1 mM complete inhibition | Glycine max | |
iodoacetamide | - |
Glycine max | |
Iodosobenzoate | - |
Glycine max | |
malonate | - |
Glycine max | |
NEM | - |
Glycine max | |
Pb2+ | up to 10 mM complete inhibibition | Glycine max | |
PCMB | - |
Glycine max | |
propionate | - |
Glycine max | |
protoporphyrin IX | - |
Glycine max | |
sodium arsenite | - |
Glycine max | |
succinate | above 0.2 M | Glycine max | |
Zn2+ | up to 1 mM complete inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
ATP | soluble enzyme | Glycine max | |
14 | - |
succinate | soluble enzyme | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km: 1.5 mM, soluble enzyme | Glycine max | |
Mg2+ | absolute requirement for either Mg2+ or Mn2+, optimal concentration of Mg2+ is 5 mM | Glycine max | |
Mn2+ | absolute requirement for either Mg2+ or Mn2+, Km: 3.5 mM | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | enzyme is involved in the initial step of porphyrin biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
stable for several months in the frozen state | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + succinate + CoA | specific for ATP | Glycine max | ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | specific for succinate | Glycine max | ADP + phosphate + succinyl-CoA | - |
? | |
additional information | enzyme is involved in the initial step of porphyrin biosynthesis | Glycine max | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.3 | - |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9 | pH 7.1: about 75% of maximal activity, pH 9.0: about 70% of maximal activity | Glycine max |