Cloned (Comment) | Organism |
---|---|
alpha and beta subunits, expression of His-tagged proteins in Escherichia coli strain BL21(DE3), phylogenetic analysis of SCS subunits | Blastocystis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme is located in a mitochondrion-like organelle | Blastocystis sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Blastocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
2 * 32500, alphabeta, SDS-PAGE, 1 * 33400, alpha-subunit, + 1 * 45100, beta-subunit, sequence calculation | Blastocystis sp. |
33400 | - |
2 * 32500, alphabeta, SDS-PAGE, 1 * 33400, alpha-subunit, + 1 * 45100, beta-subunit, sequence calculation | Blastocystis sp. |
45100 | - |
2 * 32500, alphabeta, SDS-PAGE, 1 * 33400, alpha-subunit, + 1 * 45100, beta-subunit, sequence calculation | Blastocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Blastocystis sp. | Blastocystis succinyl-CoA synthetase is a tricarboxylic acid cycle enzyme that conserves energy by substrate-level phosphorylation. In the absence of a classic mitochondrial electron transport chain, it is likely to be one of the main ATP producing enzymes in this parasite | ADP + phosphate + acetyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastocystis sp. | - |
human isolate, strain DMP/02-328, ATCC 50177 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged proteins from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Blastocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Blastocystis succinyl-CoA synthetase is a tricarboxylic acid cycle enzyme that conserves energy by substrate-level phosphorylation. In the absence of a classic mitochondrial electron transport chain, it is likely to be one of the main ATP producing enzymes in this parasite | Blastocystis sp. | ADP + phosphate + acetyl-CoA | - |
r | |
ATP + acetate + CoA | Blastocystis SCS is ATP-specific, while both ATP and GTP fit into the Blastocystis SCS active site, GTP is destabilizes by electrostatic dipole interactions with Lys42 and Lys110, the side-chains of which lie outside the nucleotide-binding cavity, an electrostatic gatekeeper controls which ligands can enter the binding site, overview | Blastocystis sp. | ADP + phosphate + acetyl-CoA | three reaction steps in the reverse direction: 1. formation of a non-covalent enzyme-succinyl-phosphate complex and cocomitant release of CoA, 2. formation of a covalent phosphoryl-enzyme intermediate with the release of succinate, and 3. phosphorylation of ADP forming ATP | r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32500, alphabeta, SDS-PAGE, 1 * 33400, alpha-subunit, + 1 * 45100, beta-subunit, sequence calculation | Blastocystis sp. |
More | homology modelling using pig SCS, PDB code 2fp4, induced-fit docking calculations and molecular dynamics simulations, overview | Blastocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
SCS | - |
Blastocystis sp. |
Succinyl-CoA synthetase | - |
Blastocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Blastocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Blastocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | specific for, while both ATP and GTP fit into the Blastocystis SCS active site, GTP is destabilized by electrostatic dipole interactions with Lys42 and Lys110, the side-chains of which lie outside the nucleotide-binding cavity, flexible docking and molecular dynamics simulations, overview | Blastocystis sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Blastocystis sp. | beta-subunit, sequence calculation | - |
8.47 |
Blastocystis sp. | alpha-subunit, sequence calculation | - |
9.34 |