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Literature summary for 6.2.1.5 extracted from
- A novel ADP-forming succinyl-CoA synthetase in Thermococcus kodakaraensis structurally related to the archaeal nucleoside diphosphate-forming acetyl-CoA synthetases (2007), J. Biol. Chem., 282, 26963-26970.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
ATP | at 55°C | Thermococcus kodakarensis |  |
| 0.0077 | - |
ADP | at 55°C | Thermococcus kodakarensis | |
| 0.06 | - |
CoA | at 55°C | Thermococcus kodakarensis | |
| 1.4 | - |
phosphate | at 55°C | Thermococcus kodakarensis | |
| 2.8 | - |
succinate | at 55°C | Thermococcus kodakarensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
SDS-PAGE, beta subunit | Thermococcus kodakarensis |
| 50000 | - |
SDS-PAGE, alpha subunit | Thermococcus kodakarensis |
| 160000 | - |
circa 160000 Da, gel filtration | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Resource Q column chromatography, Resource ISO column chromatography, and Superdex 200 HR 10/30 gel filtration | Thermococcus kodakarensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
cell extract | Thermococcus kodakarensis |
| 39.4 | - |
after 221fold purification | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adipate + CoA | 59% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + adipyl-CoA | - |
r | |
| ATP + butyrate + CoA | 48% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + butyryl-CoA | - |
r | |
| ATP + glutarate + CoA | 121% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + glutaryl-CoA | - |
r | |
| ATP + oxalate + CoA | 9% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + oxalyl-CoA | - |
r | |
| ATP + propionate + CoA | 10% activity compared to succinate | Thermococcus kodakarensis | ADP + phosphate + propionyl-CoA | - |
r | |
| ATP + succinate + CoA | - |
Thermococcus kodakarensis | ADP + phosphate + succinyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 50000 + 2 * 27000, gel filtration | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP-forming succinyl-CoA synthetase | - |
Thermococcus kodakarensis |
| SCS | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 80 | - |
Thermococcus kodakarensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
phosphate | at 55°C | Thermococcus kodakarensis | |
| 22 | - |
ADP | at 55°C | Thermococcus kodakarensis | |
| 23 | - |
ATP | at 55°C | Thermococcus kodakarensis | |
| 25 | - |
CoA | at 55°C | Thermococcus kodakarensis | |
| 29 | - |
succinate | at 55°C | Thermococcus kodakarensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Thermococcus kodakarensis |
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Release 2023.1 (January 2023)
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