Literature summary for 6.2.1.5 extracted from

Hidber, E.; Brownie, E.R.; Hayakawa, K.; Fraser, M.E.
Participation of Cys123alpha of Escherichia coli succinyl-CoA synthetase in catalysis (2007), Acta Crystallogr. Sect. D, D63, 876-884.

Search for more literature for 6.2.1.5

Protein Variants

Protein Variants	Comment	Organism
C123ALPHAA	lower catalytic efficiency compared to the wild type enzyme	Escherichia coli
C123ALPHAS	lower catalytic efficiency compared to the wild type enzyme	Escherichia coli
C123ALPHAT	lower catalytic efficiency compared to the wild type enzyme	Escherichia coli
C123ALPHAV	lower catalytic efficiency compared to the wild type enzyme	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	ATP	wild type enzyme, apparent value	Escherichia coli
0.09	-	ATP	mutant enzyme C123ALPHAV, apparent value	Escherichia coli
0.1	-	ATP	mutant enzyme C123ALPHAS, apparent value	Escherichia coli
0.1	-	ATP	mutant enzyme C123ALPHAT, apparent value	Escherichia coli
0.18	-	ATP	mutant enzyme C123ALPHAA, apparent value	Escherichia coli
0.25	-	succinate	wild type enzyme, apparent value	Escherichia coli
0.71	-	succinate	mutant enzyme C123ALPHAA, apparent value	Escherichia coli
0.8	-	succinate	mutant enzyme C123ALPHAS, apparent value	Escherichia coli
1.3	-	succinate	mutant enzyme C123ALPHAV, apparent value	Escherichia coli
3.3	-	succinate	mutant enzyme C123ALPHAT, apparent value	Escherichia coli
4	-	CoA	wild type enzyme, apparent value	Escherichia coli
11	-	CoA	mutant enzyme C123ALPHAS, apparent value	Escherichia coli
17	-	CoA	mutant enzyme C123ALPHAA, apparent value	Escherichia coli
20	-	CoA	mutant enzyme C123ALPHAV, apparent value	Escherichia coli
29	-	CoA	mutant enzyme C123ALPHAT, apparent value	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + succinate + CoA	-	Escherichia coli	ADP + phosphate + succinyl-CoA	-	r

Synonyms

Synonyms	Comment	Organism
SCS	-	Escherichia coli
Succinyl-CoA synthetase	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.3	-	succinate	mutant enzyme C123ALPHAA, apparent value	Escherichia coli
15.3	-	succinate	mutant enzyme C123ALPHAV, apparent value	Escherichia coli
29.25	-	succinate	mutant enzyme C123ALPHAS, apparent value	Escherichia coli
31	-	succinate	mutant enzyme C123ALPHAT, apparent value	Escherichia coli
35.38	-	succinate	mutant enzyme C123ALPHAA, apparent value	Escherichia coli
44.73	-	succinate	wild type enzyme, apparent value	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)