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Literature summary for 6.2.1.45 extracted from

  • Liu, H.Y.; Pfleger, C.M.
    Mutation in E1, the ubiquitin activating enzyme, reduces Drosophila lifespan and results in motor impairment (2013), PLoS ONE, 8, e32835.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genotyping-phenotyping Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information genotyping-phenotyping Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Drosophila melanogaster 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Drosophila melanogaster
-
AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
-
Drosophila melanogaster AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
?

Synonyms

Synonyms Comment Organism
E1
-
Drosophila melanogaster
Uba1
-
Drosophila melanogaster
ubiquitin activating enzyme
-
Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Drosophila melanogaster

General Information

General Information Comment Organism
malfunction identification of loss-of-function mutations in the Drosophila ubiquitin activating enzyme, the most upstream enzyme in the ubiquitin pathway. Loss of only one functional copy of E1 caused a significant reduction in adult lifespan. Rare homozygous hypomorphic E1 mutants reach adulthood, but mutants exhibit further reduced lifespan and show inappropriate Ras activation in the brain. Removing just one functional copy of Ras restores the lifespan of heterozygous E1 mutants to that of wild-type flies and increase the survival of homozygous E1 mutants. E1 homozygous mutants also show severe motor impairment involved in early mortality. Phenotypes, detailed overview Drosophila melanogaster