Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The recombinant enzyme is modified by acetylation at Lys594 and is partly inactive | Pyrobaculum neutrophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
4-hydroxybutanoate | pH 7.8, 85°C | Pyrobaculum neutrophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71356 | - |
x * 71356, calculated from sequence | Pyrobaculum neutrophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-hydroxybutanoate + CoA | Pyrobaculum neutrophilum | the enzyme is involved in autotrophic carbon fixation | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | Pyrobaculum neutrophilum DSM 2338 | the enzyme is involved in autotrophic carbon fixation | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum neutrophilum | B1YBY4 | - |
- |
Pyrobaculum neutrophilum DSM 2338 | B1YBY4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum neutrophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
pH 7.8, 85°C | Pyrobaculum neutrophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxybutyrate + CoA | 8% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + 3-hydroxybutyryl-CoA | - |
? | |
ATP + 3-hydroxybutyrate + CoA | 8% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + 3-hydroxybutyryl-CoA | - |
? | |
ATP + 3-hydroxypropionate + CoA | 9% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + 3-hydroxypropionyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | - |
Pyrobaculum neutrophilum | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | - |
Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + acetate + CoA | 13% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | 13% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + crotonate + CoA | 22% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + crotonyl-CoA | - |
? | |
ATP + crotonate + CoA | 22% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + crotonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71356, calculated from sequence | Pyrobaculum neutrophilum |
Synonyms | Comment | Organism |
---|---|---|
Tneu_0420 | locus name | Pyrobaculum neutrophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Pyrobaculum neutrophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Pyrobaculum neutrophilum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum |