Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.40 extracted from

  • Ramos-Vera, W.H.; Weiss, M.; Strittmatter, E.; Kockelkorn, D.; Fuchs, G.
    Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota (2011), J. Bacteriol., 193, 1201-1211.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The recombinant enzyme is modified by acetylation at Lys594 and is partly inactive Pyrobaculum neutrophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 4-hydroxybutanoate pH 7.8, 85°C Pyrobaculum neutrophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71356
-
 x * 71356, calculated from sequence Pyrobaculum neutrophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-hydroxybutanoate + CoA Pyrobaculum neutrophilum the enzyme is involved in autotrophic carbon fixation AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA Pyrobaculum neutrophilum DSM 2338 the enzyme is involved in autotrophic carbon fixation AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum neutrophilum B1YBY4
-
-
Pyrobaculum neutrophilum DSM 2338 B1YBY4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum neutrophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
 pH 7.8, 85°C Pyrobaculum neutrophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-hydroxybutyrate + CoA 8% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum AMP + diphosphate + 3-hydroxybutyryl-CoA
-
 ?
ATP + 3-hydroxybutyrate + CoA 8% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum DSM 2338 AMP + diphosphate + 3-hydroxybutyryl-CoA
-
 ?
ATP + 3-hydroxypropionate + CoA 9% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum AMP + diphosphate + 3-hydroxypropionyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA
-
 Pyrobaculum neutrophilum AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA the enzyme is involved in autotrophic carbon fixation Pyrobaculum neutrophilum AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA
-
 Pyrobaculum neutrophilum DSM 2338 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA the enzyme is involved in autotrophic carbon fixation Pyrobaculum neutrophilum DSM 2338 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + acetate + CoA 13% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA 13% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum DSM 2338 AMP + diphosphate + acetyl-CoA
-
 ?
ATP + crotonate + CoA 22% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum AMP + diphosphate + crotonyl-CoA
-
 ?
ATP + crotonate + CoA 22% of the activity compared to 4-hydroxybutanoate Pyrobaculum neutrophilum DSM 2338 AMP + diphosphate + crotonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 71356, calculated from sequence Pyrobaculum neutrophilum

Synonyms

Synonyms Comment Organism
Tneu_0420 locus name Pyrobaculum neutrophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Pyrobaculum neutrophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Pyrobaculum neutrophilum

General Information

General Information Comment Organism
physiological function the enzyme is involved in autotrophic carbon fixation Pyrobaculum neutrophilum