BRENDA - Enzyme Database show
show all sequences of 6.2.1.4

Cloning, expression, purification, crystallization and preliminary X-ray analysis of Thermus aquaticus succinyl-CoA synthetase

Joyce, M.A.; Brownie, E.R.; Hayakawa, K.; Fraser, M.E.; Acta Crystallogr. Sect. F 63, 399-402 (2007)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
hanging drop vapour diffusion method using using 10% polyethylene glycol 3350, 100 mM 4-morpholinoethanesulfonic acid (pH 6.4 for 1 M solution) and 200 mM KCl and a protein solution containing 5 mM GDP and 10 mM MnCl2
Thermus aquaticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Thermus aquaticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24520
-
calculated from amino acid sequence
Thermus aquaticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermus aquaticus
-
-
-
Purification (Commentary)
Commentary
Organism
Sephadex G-50 coarse column gel filtration, Q Sepharose FF column chromatography, and Sephacryl S200 SF gel filtration
Thermus aquaticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + succinate + CoA
-
671128
Thermus aquaticus
GDP + phosphate + succinyl-CoA
-
-
-
r
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapour diffusion method using using 10% polyethylene glycol 3350, 100 mM 4-morpholinoethanesulfonic acid (pH 6.4 for 1 M solution) and 200 mM KCl and a protein solution containing 5 mM GDP and 10 mM MnCl2
Thermus aquaticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Thermus aquaticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24520
-
calculated from amino acid sequence
Thermus aquaticus
Purification (Commentary) (protein specific)
Commentary
Organism
Sephadex G-50 coarse column gel filtration, Q Sepharose FF column chromatography, and Sephacryl S200 SF gel filtration
Thermus aquaticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GTP + succinate + CoA
-
671128
Thermus aquaticus
GDP + phosphate + succinyl-CoA
-
-
-
r
Other publictions for EC 6.2.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744369
Vashisht
Engineering nucleotide specif ...
Blastocystis hominis, Blastocystis hominis NandII
Biochemistry
56
534-542
2017
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5
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743947
Huang
Structural basis for the bind ...
Sus scrofa
Acta Crystallogr. Sect. D
72
912-921
2016
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744272
Kacso
Two transgenic mouse models f ...
Mus musculus
Biochem. J.
473
3463-3485
2016
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745500
Carrozzo
Succinate-CoA ligase deficien ...
Homo sapiens
J. Inherit. Metab. Dis.
39
243-252
2016
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1
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746174
Gomes
Succinyl-CoA synthetase New a ...
Bartonella bacilliformis
PLoS Negl. Trop. Dis.
10
e0004989
2016
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743957
Huang
Structure of GTP-specific suc ...
Sus scrofa
Acta Crystallogr. Sect. F
71
1067-1071
2015
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1
1
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745246
Dobolyi
Localization of SUCLA2 and SU ...
Homo sapiens
J. Bioenerg. Biomembr.
47
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2015
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745498
Luis
Valproyl-CoA inhibits the act ...
Homo sapiens
J. Inherit. Metab. Dis.
37
353-357
2014
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1
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727203
Li
Identification of the kinetic ...
Sus scrofa
Biosci. Rep.
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145-163
2013
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728078
Luis
Valproyl-CoA inhibits the acti ...
Homo sapiens
J. Inherit. Metab. Dis.
37
353-357
2013
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3
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1
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1
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726583
Joyce
Biochemical and structural cha ...
Thermus aquaticus
Acta Crystallogr. Sect. D
68
751-762
2012
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714342
Miller
The interplay between SUCLA2, ...
Homo sapiens
Biochim. Biophys. Acta
1812
625-629
2011
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3
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715381
Schuermann
Novel reaction of succinyl coe ...
Advenella mimigardefordensis, Advenella mimigardefordensis DPN7T
J. Bacteriol.
193
3078-3089
2011
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16
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16
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687185
Fleige
Localisation of gluconeogenesi ...
Toxoplasma gondii
Int. J. Parasitol.
38
1121-1132
2008
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690760
Tsugawa
Alpha-ketoglutarate oxidoreduc ...
Helicobacter pylori
Biochem. Biophys. Res. Commun.
376
46-51
2008
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693488
Ostergaard
Disorders caused by deficiency ...
Homo sapiens
J. Inherit. Metab. Dis.
31
226-229
2008
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5
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8
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2
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2
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671128
Joyce
Cloning, expression, purificat ...
Thermus aquaticus
Acta Crystallogr. Sect. F
63
399-402
2007
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672980
Kibbey
Mitochondrial GTP regulates gl ...
Rattus norvegicus
Cell Metab.
5
253-264
2007
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674529
Fraser
Interactions of GTP with the A ...
Sus scrofa
J. Biol. Chem.
281
11058-11065
2006
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1
1
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662256
Lambeth
Expression of two succinyl-CoA ...
Rattus norvegicus
J. Biol. Chem.
279
36621-36624
2004
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650949
Kowluru
Adenine and guanine nucleotide ...
Rattus norvegicus
Diabetologia
44
89-94
2001
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652820
Fraser
Phosphorylated and dephosphory ...
Sus scrofa
J. Mol. Biol.
299
1325-1339
2000
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651897
Johnson
Characterization of the ATP- a ...
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273
27573-27579
1998
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6
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6
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1
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1
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793
Ryan D.G.; Lin
Mutually exclusive splicing ge ...
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795
Birney
Cloning and expression of the ...
Dictyostelium discoideum
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319
93-101
1995
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