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Literature summary for 6.2.1.33 extracted from

  • Chang, K.H.; Liang, P.H.; Beck, W.; Scholten, J.D.; Dunaway-Mariano, D.
    Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3 (1992), Biochemistry, 31, 5605-5610.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 4-Chlorobenzoate
-
 Pseudomonas sp.
0.08
-
 CoA
-
 Pseudomonas sp.
0.1
-
 ATP
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Pseudomonas sp.
Mg2+ required Pseudomonas sp.
Mn2+ activates Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 2 * 57000, SDS-PAGE Pseudomonas sp.
114000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Bromobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Chlorobenzoate + CoA + ATP
-
 Pseudomonas sp. CBS-3 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Iodobenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
4-Methylbenzoate + CoA + ATP
-
 Pseudomonas sp. 4-Methylbenzoyl-CoA + AMP + diphosphate
-
 ?
ATP + 4-chlorobenzoate + CoA ATP in form of MgATP2- Pseudomonas sp. AMP + diphosphate + 4-chlorobenzoyl-CoA
-
 ?
Benzoate + CoA + ATP
-
 Pseudomonas sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57000, SDS-PAGE Pseudomonas sp.