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Literature summary for 6.2.1.32 extracted from

  • Lee, H.L.; Kim, S.Y.; Kim, E.J.; Han, D.Y.; Kim, B.G.; Ahn, J.H.
    Synthesis of methylated anthranilate derivatives using engineered strains of Escherichia coli (2019), J. Microbiol. Biotechnol., 29, 839-844 .
    View publication on PubMed

Application

Application Comment Organism
synthesis three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Eschetrichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4X3
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Synonyms

Synonyms Comment Organism
PqsA
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Pseudomonas aeruginosa