Cloned (Comment) | Organism |
---|---|
gene paaK1 is encoded in the paaFZJGIK1 operon located on the chromosome 1 | Burkholderia cenocepacia |
gene paaK2 is encoded in the paaHK2paaG operon located on the chromosome 2 | Burkholderia cenocepacia |
Protein Variants | Comment | Organism |
---|---|---|
additional information | contruction of paak1 deletion mutant IAI1, insertional mutagenesis of paaK1, which encodes phenylacetate-CoA ligase, does not result in a phenylacetate-conditional growth | Burkholderia cenocepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | Burkholderia cenocepacia | - |
AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | Burkholderia cenocepacia DSM 16553 | - |
AMP + diphosphate + phenylacetyl-CoA | - |
? | |
additional information | Burkholderia cenocepacia | isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 | ? | - |
? | |
additional information | Burkholderia cenocepacia DSM 16553 | isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cenocepacia | B4E7B5 | gene paaK1 | - |
Burkholderia cenocepacia | B4EL89 | gene paaK2 | - |
Burkholderia cenocepacia DSM 16553 | B4E7B5 | gene paaK1 | - |
Burkholderia cenocepacia DSM 16553 | B4EL89 | gene paaK2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Burkholderia cenocepacia utilizes 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid as sole carbon sources, but not exclusively through phenylacetate degradation | Burkholderia cenocepacia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | - |
Burkholderia cenocepacia | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | - |
Burkholderia cenocepacia DSM 16553 | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
additional information | isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 | Burkholderia cenocepacia | ? | - |
? | |
additional information | isozyme PaaK1 is more active than isozyme PaaK2, the larger binding pocket of PaaK1 can accommodate hydroxylated phenylacetate-derived molecules such as 3-hydroxyphenylacetic acid and 4-hydroxyphenylacetic acid, PaaK1 shows higher activity and broader substrate specificity than PaaK2 | Burkholderia cenocepacia DSM 16553 | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Burkholderia cenocepacia |
Organism | Comment | Expression |
---|---|---|
Burkholderia cenocepacia | 3-hydroxyphenylacetic acid induces the phenylacetic acid degradation pathway | up |
Burkholderia cenocepacia | 3-hydroxyphenylacetic acid induces the phenylacetic acid degradation pathway, while 4-hydroxyphenylacetic acid does not | up |
General Information | Comment | Organism |
---|---|---|
additional information | insertional mutagenesis of paaK1, which encodes phenylacetate-CoA ligase, does not result in a phenylacetate-conditional growth probably due to the presence of a putative paralogue gene paaK2 | Burkholderia cenocepacia |
additional information | insertional mutagenesis of paaK1, which encodes phenylacetate-CoA ligase, does not result in a phenylacetate-conditional growth probably due to the presence of a putative paralogue gene paaK2. The paaK1 deletion mutant IAI1 does not show any growth-defective phenotype in phenylacetate | Burkholderia cenocepacia |
physiological function | isozyme PaaK1 does not play a distinct role in pathogenesis of Burkholderia cenocepacia in Caenorhabditis elegans, although the catabolic pathway for phenylacetic acid degradation is a requirement for full pathogenesis | Burkholderia cenocepacia |