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Literature summary for 6.2.1.30 extracted from

  • del Peso-Santos, T.; Shingler, V.; Perera, J.
    The styrene-responsive StyS/StyR regulation system controls expression of an auxiliary phenylacetyl-coenzyme A ligase: implications for rapid metabolic coupling of the styrene upper- and lower-degradative pathways (2008), Mol. Microbiol., 69, 317-330.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes paaF, DNA and amino acid sequence determination, strain Y2 is unique in having three paaF genes located within two complete copies of the paa gene clusters. Expression of both paaF and paaF3 is controlled by the PaaX repressor, expression analysis, overview, gene paaF2, adjacent to the StyS/StyR regulatory genes, belongs to the StyR regulon and is not subject to repression by PaaX, and the PpaaF2 promoter region contains a putative StyR binding site Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of paaF mutants Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Pseudomonas sp. the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA Pseudomonas sp. Y2 the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA AMP + diphosphate + phenylacetyl-CoA
-
?
additional information Pseudomonas sp. regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase ?
-
?
additional information Pseudomonas sp. Y2 regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
nd diverse derivatives, overview, genes paaF1, paaF2, and paaF3
-
Pseudomonas sp. Y2
-
nd diverse derivatives, overview, genes paaF1, paaF2, and paaF3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phenylacetate + CoA
-
Pseudomonas sp. AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA Pseudomonas sp. AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA
-
Pseudomonas sp. Y2 AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA Pseudomonas sp. Y2 AMP + diphosphate + phenylacetyl-CoA
-
?
additional information regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase Pseudomonas sp. ?
-
?
additional information regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase Pseudomonas sp. Y2 ?
-
?

Synonyms

Synonyms Comment Organism
PA-CoA ligase
-
Pseudomonas sp.
PaaF2
-
Pseudomonas sp.
phenylacetyl-coenzyme A ligase
-
Pseudomonas sp.
styrene-specific PA-CoA ligase
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
Pseudomonas sp.