Cloned (Comment) | Organism |
---|---|
genes paaF, DNA and amino acid sequence determination, strain Y2 is unique in having three paaF genes located within two complete copies of the paa gene clusters. Expression of both paaF and paaF3 is controlled by the PaaX repressor, expression analysis, overview, gene paaF2, adjacent to the StyS/StyR regulatory genes, belongs to the StyR regulon and is not subject to repression by PaaX, and the PpaaF2 promoter region contains a putative StyR binding site | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of paaF mutants | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | Pseudomonas sp. | the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | Pseudomonas sp. Y2 | the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
additional information | Pseudomonas sp. | regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase | ? | - |
? | |
additional information | Pseudomonas sp. Y2 | regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
nd diverse derivatives, overview, genes paaF1, paaF2, and paaF3 | - |
Pseudomonas sp. Y2 | - |
nd diverse derivatives, overview, genes paaF1, paaF2, and paaF3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phenylacetate + CoA | - |
Pseudomonas sp. | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA | Pseudomonas sp. | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | - |
Pseudomonas sp. Y2 | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
ATP + phenylacetate + CoA | the first step of this phenylacetate degradation pathway is catalysed by paaF-encoded phenylacetyl-CoA ligases that produce phenylacetyl-CoA | Pseudomonas sp. Y2 | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
additional information | regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase | Pseudomonas sp. | ? | - |
? | |
additional information | regulation of the paaF2 gene, the otherwise metabolically redundant PaaF2 auxiliary enzyme provides a system for rapid co-ordinate de-repression of the two sets of catabolic genes required for styrene degradation, overview. paaF2-encoded phenylacetate-CoA ligase activity is only induced in response to styrene in vivo and can be considered a styrene-specific phenylacetate-CoA ligase | Pseudomonas sp. Y2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PA-CoA ligase | - |
Pseudomonas sp. |
PaaF2 | - |
Pseudomonas sp. |
phenylacetyl-coenzyme A ligase | - |
Pseudomonas sp. |
styrene-specific PA-CoA ligase | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas sp. |