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Literature summary for 6.2.1.30 extracted from

  • Wang, F.Q.; Liu, J.; Dai, M.; Ren, Z.H.; Su, C.Y.; He, J.G.
    Molecular cloning and functional identification of a novel phenylacetyl-CoA ligase gene from Penicillium chrysogenum (2007), Biochem. Biophys. Res. Commun., 360, 453-458.
    View publication on PubMed
Search for more literature for 6.2.1.30

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme has a peroxisomal targeting signal on its C-terminal Penicillium chrysogenum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62638
-
 x * 62638, calculated from sequence Penicillium chrysogenum
64000
-
 x * 64000, SDS-PAGE Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Penicillium chrysogenum PhlB might participate in the activation of phenylacetic acid during penicillin biosynthesis in Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum A7KUK6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
additional information the expression level of phlB in the penicillin producing medium supplemented with phenylacetic acid, the side chain precursor of penicillin G, is about 2.5fold higher than that in medium without phenylacetic acid Penicillium chrysogenum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phenylacetate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
 ?
ATP + phenylacetate + CoA PhlB might participate in the activation of phenylacetic acid during penicillin biosynthesis in Penicillium chrysogenum Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Penicillium chrysogenum
? x * 62638, calculated from sequence Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
phlB
-
 Penicillium chrysogenum

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium chrysogenum calculated from sequence
-
 8.34