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Literature summary for 6.2.1.30 extracted from

  • Mohamed, M.E.S.
    Biochemical and molecular characterization of phenylacetate-coenzyme A ligase, an enzyme catalyzing the first step in aerobic metabolism of phenylacetic acid in Azoarcus evansii (2000), J. Bacteriol., 182, 286-294.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Aromatoleum evansii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, more than 80% inhibition Aromatoleum evansii
iodoacetamide
-
Aromatoleum evansii
NaF 2 mM; 2 mM, 84% loss of activity Aromatoleum evansii
NEM
-
Aromatoleum evansii
Ni2+ 1 mM, more than 80% inhibition Aromatoleum evansii
Zn2+ 1 mM, more than 80% inhibition Aromatoleum evansii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
phenylacetate 37°C Aromatoleum evansii
0.045
-
CoA 37°C Aromatoleum evansii
0.06
-
ATP 37°C Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aromatoleum evansii
Mn2+ 36% of the activation with Mg2+ Aromatoleum evansii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48750
-
x * 48750, calculation from nucleotide sequence Aromatoleum evansii
50000
-
1 * 50000, SDS-PAGE Aromatoleum evansii
52000
-
gel filtration Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + phenylacetate + CoA Aromatoleum evansii first enzyme involved in the aerobic catabolism of phenylacetic acid ?
-
?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aromatoleum evansii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000076
-
maximal specific activity in growing cultures Aromatoleum evansii
0.48
-
-
Aromatoleum evansii

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, activity is retained for 2 months without remarkable loss of activity Aromatoleum evansii
4°C, 10% glycerol, 65% of the activity is recovered after 72 h Aromatoleum evansii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phenylacetate + CoA
-
Aromatoleum evansii AMP + diphosphate + phenylacetyl-CoA
-
?
ATP + phenylacetate + CoA first enzyme involved in the aerobic catabolism of phenylacetic acid Aromatoleum evansii ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Aromatoleum evansii
monomer x * 48750, calculation from nucleotide sequence Aromatoleum evansii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
ATP 37°C Aromatoleum evansii
40
-
CoA 37°C Aromatoleum evansii
40
-
phenylacetate 37°C Aromatoleum evansii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Aromatoleum evansii

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 9: 55% of maximal activity, pH 9.0: 50% of maximal activity Aromatoleum evansii

pI Value

Organism Comment pI Value Maximum pI Value
Aromatoleum evansii isoelectric focusing
-
6.1