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Literature summary for 6.2.1.3 extracted from

  • Kameda, K.; Nunn, W.D.
    Purification and characterization of acyl coenzyme A synthetase from Escherichi coli (1981), J. Biol. Chem., 256, 5702-5707.
    View publication on PubMed
Search for more literature for 6.2.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 Undecanoate
-
 Escherichia coli
0.0015
-
 palmitoleate
-
 Escherichia coli
0.0016
-
 laurate
-
 Escherichia coli
0.0022
-
 Pentadecanoate
-
 Escherichia coli
0.0024
-
 myristate palmitate Escherichia coli
0.0034
-
 tridecanoate
-
 Escherichia coli
0.0043
-
 Decanoate
-
 Escherichia coli
0.0085
-
 oleate
-
 Escherichia coli
0.0089
-
 stearate
-
 Escherichia coli
0.0102
-
 heptadecanoate
-
 Escherichia coli
0.0112
-
 hexanoate
-
 Escherichia coli
0.026
-
 Heptanoate
-
 Escherichia coli
0.033
-
 ATP nonanoate Escherichia coli
0.0408
-
 Octanoate
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * or 3 * 47000, SDS-PAGE Escherichia coli
47000
-
 3 * or 2 * 47000, SDS-PAGE Escherichia coli
130000
-
 gel filtration, gradient PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.24
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, indefinitely stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + decanoate + CoA
-
 Escherichia coli AMP + diphosphate + decanoyl-CoA
-
 ?
ATP + heptadecanoate + CoA
-
 Escherichia coli AMP + diphosphate + heptadecanoyl-CoA
-
 ?
ATP + heptanoate + CoA
-
 Escherichia coli AMP + diphosphate + heptanoyl-CoA
-
 ?
ATP + hexanoate + CoA
-
 Escherichia coli AMP + diphosphate + hexanoyl-CoA
-
 ?
ATP + laurate + CoA
-
 Escherichia coli AMP + diphosphate + lauroyl-CoA
-
 ?
ATP + long-chain carboxylic acid + CoA active on fatty acids with chain length of 6 to 18 carbon atoms, and a maximum activity with laurate. Low affinity for the fatty acids C6 to C8 Escherichia coli AMP + diphosphate + long-chain acyl-CoA
-
 ?
ATP + myristate + CoA
-
 Escherichia coli AMP + diphosphate + myristoyl-CoA
-
 ?
ATP + nonanoate + CoA
-
 Escherichia coli AMP + diphosphate + nonanoyl-CoA
-
 ?
ATP + octanoate + CoA
-
 Escherichia coli AMP + diphosphate + octanoyl-CoA
-
 ?
ATP + oleate + CoA
-
 Escherichia coli AMP + diphosphate + oleoyl-CoA
-
 ?
ATP + palmitate + CoA
-
 Escherichia coli AMP + diphosphate + palmitoyl-CoA
-
 ?
ATP + palmitoleate + CoA
-
 Escherichia coli AMP + diphosphate + palmitoleoyl-CoA
-
 ?
ATP + pentadecanoate + CoA
-
 Escherichia coli AMP + diphosphate + pentadecanoyl-CoA
-
 ?
ATP + stearate + CoA
-
 Escherichia coli AMP + diphosphate + stearoyl-CoA
-
 ?
ATP + tridecanoate + CoA
-
 Escherichia coli AMP + diphosphate + tridecanoyl-CoA
-
 ?
ATP + undecanoate + CoA
-
 Escherichia coli AMP + diphosphate + undecanoyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * or 3 * 47000, SDS-PAGE Escherichia coli
trimer 3 * or 2 * 47000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 complete inactivation within 24 h Escherichia coli