Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | absolute requirement, acyl-CoA synthetase II | Yarrowia lipolytica | |
phosphatidylethanolamine | slight stimulation, acyl-CoA synthetase II | Yarrowia lipolytica | |
phosphatidylserine | slight stimulation, acyl-CoA synthetase II | Yarrowia lipolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
CoA | - |
Yarrowia lipolytica | |
0.11 | - |
dephospho-CoA | - |
Yarrowia lipolytica | |
0.5 | - |
ATP | N6-etheno-CoA | Yarrowia lipolytica | |
2.5 | - |
pantetheine | - |
Yarrowia lipolytica | |
3.3 | - |
4'-phosphopantetheine | - |
Yarrowia lipolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microbody | enzyme form acyl-CoA synthetase II | Yarrowia lipolytica | 42579 | - |
microsome | enzyme form acyl-CoA synthetase I | Yarrowia lipolytica | - |
- |
mitochondrion | enzyme form acyl-CoA synthetase I | Yarrowia lipolytica | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I | Yarrowia lipolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + long-chain carboxylic acid + CoA | Yarrowia lipolytica | acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | - |
2 enzyme forms: long-chain acyl-CoA synthetase I and long-chain acyl-CoA synthetase II | - |
Purification (Comment) | Organism |
---|---|
- |
Yarrowia lipolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.8 | - |
- |
Yarrowia lipolytica |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 1 month | Yarrowia lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + decanoate + CoA | ineffective | Yarrowia lipolytica | AMP + diphosphate + decanoyl-CoA | - |
? | |
ATP + fatty acid + 4'-phosphopantetheine | - |
Yarrowia lipolytica | AMP + diphosphate + acyl-4'-phosphopantetheine | - |
? | |
ATP + fatty acid + dephospho-CoA | - |
Yarrowia lipolytica | AMP + diphosphate + acyl-dephospho-CoA | - |
? | |
ATP + fatty acid + N6-etheno-CoA | - |
Yarrowia lipolytica | AMP + diphosphate + acyl-N6-etheno-CoA | - |
? | |
ATP + fatty acid + pantetheine | - |
Yarrowia lipolytica | AMP + diphosphate + acyl-pantetheine | - |
? | |
ATP + long-chain carboxylic acid + CoA | utilizes straight-chain fatty acids with 14-18 carbon atoms regardless of the degree of unsaturation. Straight-chain fatty acids containing more than 18 or fewer than 14 carbon atoms, as well as 16-hydroxypalmitic acid and hexadecanedioic acid are ineffective. Acyl-CoA synthetase II has a broader fatty acid specificity than acyl-CoA synthetase I. The effective substrates include long-chain dicarboxylic acids | Yarrowia lipolytica | AMP + diphosphate + long-chain acyl-CoA | - |
? | |
ATP + long-chain carboxylic acid + CoA | acyl-CoA synthetase I is responsible for the production of long-chain acyl-CoA that is utilized solely for the synthesis of cellular lipids, while the acyl-CoA synthetase II provides long-chain acyl-CoA that is exclusively degraded via beta-oxidation | Yarrowia lipolytica | ? | - |
? | |
ATP + octanoate + CoA | ineffective | Yarrowia lipolytica | AMP + diphosphate + octanoyl-CoA | - |
? | |
ATP + oleate + CoA | - |
Yarrowia lipolytica | AMP + diphosphate + oleoyl-CoA | - |
? | |
ATP + palmitate + CoA | - |
Yarrowia lipolytica | AMP + diphosphate + palmitoyl-CoA | - |
? | |
dATP + fatty acid + CoA | - |
Yarrowia lipolytica | dAMP + diphosphate + acyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, SDS-PAGE, enzyme form acyl-CoA synthetase I | Yarrowia lipolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9.6 | - |
Yarrowia lipolytica |