Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a long-chain fatty acid + CoA | Mycobacterium tuberculosis | - |
AMP + diphosphate + a long-chain acyl-CoA | - |
? | |
ATP + a long-chain fatty acid + CoA | Mycobacterium tuberculosis H37Rv | - |
AMP + diphosphate + a long-chain acyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a long-chain fatty acid + CoA | - |
Mycobacterium tuberculosis | AMP + diphosphate + a long-chain acyl-CoA | - |
? | |
ATP + a long-chain fatty acid + CoA | - |
Mycobacterium tuberculosis H37Rv | AMP + diphosphate + a long-chain acyl-CoA | - |
? | |
ATP + laurate + CoA | high activity | Mycobacterium tuberculosis | AMP + diphosphate + lauroyl-CoA | - |
? | |
ATP + laurate + CoA | high activity | Mycobacterium tuberculosis H37Rv | AMP + diphosphate + lauroyl-CoA | - |
? | |
ATP + oleate + CoA | high activity | Mycobacterium tuberculosis | AMP + diphosphate + oleoyl-CoA | - |
? | |
ATP + oleate + CoA | high activity | Mycobacterium tuberculosis H37Rv | AMP + diphosphate + oleoyl-CoA | - |
? | |
ATP + palmitate + CoA | highest activity | Mycobacterium tuberculosis | AMP + diphosphate + palmitoyl-CoA | - |
? | |
ATP + palmitate + CoA | highest activity | Mycobacterium tuberculosis H37Rv | AMP + diphosphate + palmitoyl-CoA | - |
? | |
additional information | the enzyme shows very low activity with acetate, propionate, butyrate, valerate, and caprylate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme shows very low activity with acetate, propionate, butyrate, valerate, and caprylate | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FadD2 | - |
Mycobacterium tuberculosis |
long-chain fatty acyl coenzyme A ligase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme deletion results in enhanced susceptibility to pyrazinoic acid and rifampin but not to isoniazid or ethambutol | Mycobacterium tuberculosis |