Cloned (Comment) | Organism |
---|---|
menE, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-deoxy-5'-[([(1E)-5-[2-(methoxycarbonyl)phenyl]-5-oxopent-1-en-1-yl]sulfonyl)amino]adenosine | mechanism of covalent inhibition | Mycobacterium tuberculosis | |
5'-deoxy-5'-[([(1E)-5-[2-(methoxycarbonyl)phenyl]hexa-1,5-dien-1-yl]sulfonyl)amino]adenosine | mechanism of covalent inhibition | Mycobacterium tuberculosis | |
5'-deoxy-5'-[([4-[2-(methoxycarbonyl)phenyl]-4-oxobutanoyl]sulfamoyl)amino]adenosine | mechanism of covalent inhibition | Mycobacterium tuberculosis | |
5'-deoxy-5'-[([4-[2-(methoxycarbonyl)phenyl]pent-4-enoyl]sulfamoyl)amino]adenosine | mechanism of covalent inhibition | Mycobacterium tuberculosis | |
5'-O-([4-[2-(methoxycarbonyl)phenyl]-4-oxobutanoyl]sulfamoyl)adenosine | mechanism of covalent inhibition | Mycobacterium tuberculosis | |
5'-O-([4-[2-(methoxycarbonyl)phenyl]pent-4-enoyl]sulfamoyl)adenosine | mechanism of covalent inhibition | Mycobacterium tuberculosis | |
additional information | design and construction of mechanism-based inhibitors of MenE | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + o-succinyl-1-benzoate + CoA | Mycobacterium tuberculosis | a step in the bacterial biosynthesis of menaquinone from chorismate, pathway overview | AMP + diphosphate + o-succinyl-1-benzoyl-CoA | - |
? | |
additional information | Mycobacterium tuberculosis | the acyl-CoA synthetase is involved in bacterial menaquinone biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + o-succinyl-1-benzoate + CoA | a step in the bacterial biosynthesis of menaquinone from chorismate, pathway overview | Mycobacterium tuberculosis | AMP + diphosphate + o-succinyl-1-benzoyl-CoA | - |
? | |
ATP + o-succinyl-1-benzoate + CoA | a two-step reaction via o-succinyl-1-benzoyl-AMP intermediate | Mycobacterium tuberculosis | AMP + diphosphate + o-succinyl-1-benzoyl-CoA | - |
? | |
additional information | the acyl-CoA synthetase is involved in bacterial menaquinone biosynthesis | Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Acyl-CoA synthetase | - |
Mycobacterium tuberculosis |
MenE | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0057 | - |
- |
Mycobacterium tuberculosis | 5'-deoxy-5'-[([(1E)-5-[2-(methoxycarbonyl)phenyl]-5-oxopent-1-en-1-yl]sulfonyl)amino]adenosine | |
0.034 | - |
- |
Mycobacterium tuberculosis | 5'-deoxy-5'-[([4-[2-(methoxycarbonyl)phenyl]-4-oxobutanoyl]sulfamoyl)amino]adenosine | |
0.038 | - |
- |
Mycobacterium tuberculosis | 5'-O-([4-[2-(methoxycarbonyl)phenyl]-4-oxobutanoyl]sulfamoyl)adenosine | |
0.2 | - |
above | Mycobacterium tuberculosis | 5'-deoxy-5'-[([(1E)-5-[2-(methoxycarbonyl)phenyl]hexa-1,5-dien-1-yl]sulfonyl)amino]adenosine | |
0.2 | - |
above | Mycobacterium tuberculosis | 5'-deoxy-5'-[([4-[2-(methoxycarbonyl)phenyl]pent-4-enoyl]sulfamoyl)amino]adenosine | |
0.2 | - |
above | Mycobacterium tuberculosis | 5'-O-([4-[2-(methoxycarbonyl)phenyl]pent-4-enoyl]sulfamoyl)adenosine |