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Literature summary for 6.2.1.3 extracted from

  • Hall, A.M.; Wiczer, B.M.; Herrmann, T.; Stremmel, W.; Bernlohr, D.A.
    Enzymatic properties of purified murine fatty acid transport protein 4 and analysis of acyl-CoA synthetase activities in tissues from FATP4 null mice (2005), J. Biol. Chem., 280, 11948-11954.
    View publication on PubMed
Search for more literature for 6.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS1 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Triacsin C no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid, inhibits activity with palmitate with IC50 of 0.03 mM Mus musculus
troglitazone no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid up to 0.05 mM, inhibits activity with palmitate with IC50 of 0.02 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
 n-tetracosanoic acid
-
 Mus musculus
0.013
-
 palmitate
-
 Mus musculus
0.047
-
 CoA
-
 Mus musculus
1.4
-
 ATP
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus in vivo the loss of FATP4-mediated very long chain fatty acid uptake and/or esterification may underlie the null phenotype ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
intestine
-
 Mus musculus
-
liver
-
 Mus musculus
-
lung
-
 Mus musculus
-
skin
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.723
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + n-tetracosanoic acid + CoA
-
 Mus musculus AMP + diphosphate + n-tetracosanoyl-CoA
-
 ?
ATP + palmitate + CoA
-
 Mus musculus AMP + diphosphate + palmitoyl-CoA
-
 ?
additional information in vivo the loss of FATP4-mediated very long chain fatty acid uptake and/or esterification may underlie the null phenotype Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
FATP4 bifunctional enzyme, exhibiting both fatty acid transport and acyl-CoA synthetase activities Mus musculus
fatty acid transport protein bifunctional enzyme, exhibiting both fatty acid transport and acyl-CoA synthetase activities Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 substrate: palmitate Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 60% of maximal activity at pH 6.0 and pH 9.0 Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
 no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid up to 0.05 mM, inhibits activity with palmitate with IC50 of 0.02 mM Mus musculus troglitazone
0.03
-
 no effect on FATP4 acyl-CoA synthetase activity towards n-tetracosanoic acid, inhibits activity with palmitate with IC50 of 0.03 mM Mus musculus Triacsin C