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Literature summary for 6.2.1.27 extracted from

  • Barillas, W.; Beerhues, L.
    3-Hydroxybenzoate:coenzyme A ligase and 4-coumarate:coenzyme A ligase from cultured cells of Centaurium erythraea (1997), Planta, 202, 112-116.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Centaurium erythraea

Organism

Organism UniProt Comment Textmining
Centaurium erythraea
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture in the course of culture growth, the activity increases from day 6 and reaches maximum at day 10. This correlates well with the increases in the cell fresh weight and the xanthone content that starts also at day 6 Centaurium erythraea
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 17% of the activity with 3-hydroxybenzoate Centaurium erythraea AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
?
additional information no activity with 2-hydroxybenzoate, 2,3-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3,5-dihydroxybenzoate and cinnamic acid Centaurium erythraea ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30
-
Centaurium erythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Centaurium erythraea