Literature summary for 6.2.1.25 extracted from

Tiedt, O.; Mergelsberg, M.; Eisenreich, W.; Boll, M.
Promiscuous defluorinating enoyl-CoA hydratases/hydrolases allow for complete anaerobic degradation of 2-fluorobenzoate (2017), Front. Microbiol., 8, 2579-2589 .

Search for more literature for 6.2.1.25

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 2-fluorobenzoate + CoA	Thauera aromatica	activation of 2-fluorobenzoate by promiscuous AMP-forming benzoate-CoA ligase	AMP + diphosphate + 2-fluorobenzoyl-CoA	-	?
ATP + benzoate + CoA	Thauera aromatica	-	AMP + diphosphate + benzoyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Thauera aromatica	Q8GQN9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-fluorobenzoate + CoA	activation of 2-fluorobenzoate by promiscuous AMP-forming benzoate-CoA ligase	Thauera aromatica	AMP + diphosphate + 2-fluorobenzoyl-CoA	-	?
ATP + benzoate + CoA	-	Thauera aromatica	AMP + diphosphate + benzoyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
AMP-forming BCL	-	Thauera aromatica
AMP-forming benzoate-CoA ligase	-	Thauera aromatica
Bcl	-	Thauera aromatica
bclA	-	Thauera aromatica
benzoate-CoA ligase	-	Thauera aromatica

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Thauera aromatica

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in oxygen-independent defluorination and degradation of 2-fluorobenzoate to CO2 and HF, pathway overview. BCL readily activates 2-fluorobenzoate to 2-fluorobenzoyl-CoA at a rate similar to that of benzoate suggesting that 2-fluorobenzoate metabolism is initiated by CoA thioester formation	Thauera aromatica

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)