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Literature summary for 6.2.1.25 extracted from

  • Wischgoll, S.; Heintz, D.; Peters, F.; Erxleben, A.; Sarnighausen, E.; Reski, R.; Van Dorsselaer, A.; Boll, M.
    Gene clusters involved in anaerobic benzoate degradation of Geobacter metallireducens (2005), Mol. Microbiol., 58, 1238-1252.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Geobacter metallireducens

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxybenzoate competitive Geobacter metallireducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
benzoate
-
Geobacter metallireducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Geobacter metallireducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + benzoate + CoA Geobacter metallireducens the enzyme catalyzes the initial step of benzoate metabolism AMP + diphosphate + benzoyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Geobacter metallireducens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant enzyme Geobacter metallireducens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
-
Geobacter metallireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobenzoate + CoA 3% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 2-aminobenzoyl-CoA
-
?
ATP + 2-fluorobenzoate + CoA 92% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 2-fluorobenzoyl-CoA
-
?
ATP + 3-fluorobenzoate + CoA 23% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 3-fluorobenzoyl-CoA
-
?
ATP + 4-fluorobenzoate + CoA 98% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + 4-fluorobenzoyl-CoA
-
?
ATP + benzoate + CoA
-
Geobacter metallireducens AMP + diphosphate + benzoyl-CoA
-
?
ATP + benzoate + CoA the enzyme catalyzes the initial step of benzoate metabolism Geobacter metallireducens AMP + diphosphate + benzoyl-CoA
-
?
ATP + cyclohex-1-ene-1-carboxylate + CoA 13% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA
-
?
ATP + cyclohex-3-ene-1-carboxylate + CoA 40% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA
-
?
ATP + cyclohexanecarboxylate + CoA 7% of the activity with benzoate Geobacter metallireducens AMP + diphosphate + cyclohexanecarboxyl-CoA
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Geobacter metallireducens

Synonyms

Synonyms Comment Organism
BamY
-
Geobacter metallireducens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.016
-
2-Hydroxybenzoate
-
Geobacter metallireducens