Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Geobacter metallireducens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxybenzoate | competitive | Geobacter metallireducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
benzoate | - |
Geobacter metallireducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Geobacter metallireducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + benzoate + CoA | Geobacter metallireducens | the enzyme catalyzes the initial step of benzoate metabolism | AMP + diphosphate + benzoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter metallireducens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged recombinant enzyme | Geobacter metallireducens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
- |
Geobacter metallireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-aminobenzoate + CoA | 3% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + 2-aminobenzoyl-CoA | - |
? | |
ATP + 2-fluorobenzoate + CoA | 92% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + 2-fluorobenzoyl-CoA | - |
? | |
ATP + 3-fluorobenzoate + CoA | 23% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + 3-fluorobenzoyl-CoA | - |
? | |
ATP + 4-fluorobenzoate + CoA | 98% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + 4-fluorobenzoyl-CoA | - |
? | |
ATP + benzoate + CoA | - |
Geobacter metallireducens | AMP + diphosphate + benzoyl-CoA | - |
? | |
ATP + benzoate + CoA | the enzyme catalyzes the initial step of benzoate metabolism | Geobacter metallireducens | AMP + diphosphate + benzoyl-CoA | - |
? | |
ATP + cyclohex-1-ene-1-carboxylate + CoA | 13% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA | - |
? | |
ATP + cyclohex-3-ene-1-carboxylate + CoA | 40% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA | - |
? | |
ATP + cyclohexanecarboxylate + CoA | 7% of the activity with benzoate | Geobacter metallireducens | AMP + diphosphate + cyclohexanecarboxyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Geobacter metallireducens |
Synonyms | Comment | Organism |
---|---|---|
BamY | - |
Geobacter metallireducens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
2-Hydroxybenzoate | - |
Geobacter metallireducens |