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Literature summary for 6.2.1.25 extracted from
- Purification and characterization of benzoate:coenzyme A ligase from Clarkia breweri (2002), ARCH. BIOCHEM. BIOPHYS., 400, 258-264.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Clarkia breweri |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
benzoate | pH 7.5, 22°C | Clarkia breweri | |
| 0.095 | - |
ATP | pH 7.5, 22°C | Clarkia breweri | |
| 0.13 | - |
CoA | pH 7.5, 22°C | Clarkia breweri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | restores activity partially after treatment with EDTA | Clarkia breweri | |
| Mg2+ | restores activity after treatment with EDTA | Clarkia breweri | |
| Mn2+ | restores activity after treatment with EDTA | Clarkia breweri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
gel filtration | Clarkia breweri |
| 64500 | - |
Comassie blue-stained gel filtration | Clarkia breweri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarkia breweri | - |
annual California plant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE, anion-exchange, HAP, affinity on reactive red resin and anion-exchange on a Mono-Q chromatography | Clarkia breweri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
pH 7.5, 22°C | Clarkia breweri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-aminobenzoate + CoA | 50% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + 2-aminobenzoyl-CoA | - |
? | |
| ATP + 2-hydroxybenzoate + CoA | 15% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + 2-hydroxybenzoyl-CoA | - |
? | |
| ATP + 3-hydroxybenzoate + CoA | 10% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
| ATP + 4-hydroxy-3-methoxybenzoate + CoA | 15% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + 4-hydroxy-3-methoxybenzoyl-CoA | - |
? | |
| ATP + 4-hydroxybenzoate + CoA | 25% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
| ATP + benzoate + CoA | - |
Clarkia breweri | AMP + diphosphate + benzoyl-CoA | - |
? | |
| ATP + phenylacetate + CoA | 10% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + phenylacetyl-CoA | - |
? | |
| ATP + phenylpropionate + CoA | 10% of the activity relative to benzoate | Clarkia breweri | AMP + diphosphate + phenylpropionyl-CoA | - |
? | |
| additional information | no reaction with cinnamic, coumaric, ferulic, caffeic, p-aminobenzoic, octanoic, or 2,5-dihydroxybenzoic acid | Clarkia breweri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64500, gel filtration | Clarkia breweri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.4 | - |
Clarkia breweri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 9.2 | - |
Clarkia breweri |
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