Cloned (Comment) | Organism |
---|---|
gene aasS, recombinant expression of His-tagged enzyme in Escherichia coli | Vibrio harveyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas aeruginosa | |
Mg2+ | required | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a long-chain fatty acid + an [acyl-carrier protein] | Pseudomonas aeruginosa | - |
AMP + diphosphate + a long-chain acyl-[acyl-carrier protein] | - |
? | |
ATP + a long-chain fatty acid + an [acyl-carrier protein] | Vibrio harveyi | - |
AMP + diphosphate + a long-chain acyl-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Vibrio harveyi | Q00IB3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a long-chain fatty acid + an [acyl-carrier protein] | - |
Pseudomonas aeruginosa | AMP + diphosphate + a long-chain acyl-[acyl-carrier protein] | - |
? | |
ATP + a long-chain fatty acid + an [acyl-carrier protein] | - |
Vibrio harveyi | AMP + diphosphate + a long-chain acyl-[acyl-carrier protein] | - |
? | |
ATP + octanoate + [AcpP1] | recombinant holo-AcpP1 from Pseudomonas aeruginosa | Vibrio harveyi | AMP + diphosphate + octanoyl-[AcpP1] | - |
? | |
ATP + octanoate + [AcpP2] | recombinant holo-AcpP2 from Pseudomonas aeruginosa | Vibrio harveyi | AMP + diphosphate + octanoyl-[AcpP2] | - |
? | |
ATP + octanoate + [AcpP3] | recombinant holo-AcpP3 from Pseudomonas aeruginosa | Vibrio harveyi | AMP + diphosphate + octanoyl-[AcpP3] | - |
? | |
additional information | the cell-free extract of Pseudomonas aeruginosa can acylate only the holo-form of the acyl carrier protein AcpP1 for the synthesis of fatty acids, while it is inactive with acyl carrier proteins AcpP2 and AcpP3 | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme from Vibrio harveyi can acylate holo-forms of the acyl carrier proteins, AcpP1, AcpP2, and AcpP3, from Pseudomonas aeruginosa | Vibrio harveyi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AasS | - |
Pseudomonas aeruginosa |
AasS | - |
Vibrio harveyi |
Acyl-ACP synthetase | - |
Pseudomonas aeruginosa |
Acyl-ACP synthetase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas aeruginosa | |
ATP | - |
Vibrio harveyi |
General Information | Comment | Organism |
---|---|---|
malfunction | Pseudomonas aeruginosa acpP1 deletion is lethal, while disruption of acpP2 or acpP3 in the Pseudomonas aeruginosa genome allowa mutant strains to grow like the wild-type strain. AcpP1 functions in fatty acid biosynthesis with the enzyme acyl-ACP synthetase, acpP2 and acpP3 do not play roles in the fatty acid synthetic pathway. Replacement of acpP1 with Escherichia coli acpP reduces the ability of Pseudomonas aeruginosa to produce some exo-products and abolished swarming motility | Pseudomonas aeruginosa |
physiological function | only the acpP1 gene can restore growth in the Escherichia coli acpP mutant strain CY1877, while genes acpP2 and acpP3 are ineffective, neither AcpP2 nor AcpP3 can act as a substrate for synthesis of N-acylhomoserine lactones molecules in vivo. Gene acpP1 is essential for Pseudomonas aeruginosa growth | Pseudomonas aeruginosa |